caa a22 4500 46908331X CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645047 doi (NATIONALLICENCE)springer-10.1007/BF00645047 Characterization of the antigen recognized by a monoclonal antibody MN9: unique transport pathway to the equatorial segment of sperm head during spermiogenesis [Elektronische Daten] [K. Toshimori, I. Tanii, S. Araki, C. Oura] Summary: MN9, a monoclonal antibody raised against mouse spermatozoa, specifically recognizes the equatorial segment of sperm head in several mammalian species, including humans. Colloidal gold-immuno-electron microscopy of mouse spermatozoa has shown that the antigen is localized in the space between the outer and inner acrosome membranes and on the acrosome membranes at the equatorial segment. Immunoblotting after electrophoresis of spermatozoa from the cauda epididymidis has identified two immunoreactive bands: 38 kDa and 48 kDa in mouse, and 48 kDa in rat. During spermiogenesis in rat, this antigen is transported to the equatorial segment via a unique pathway, first appearing in some cisternae of the endoplasmic reticulum and in the Golgi apparatus of spermatids at around step 3. The antigen can further be found on the vesicles at thetrans-side of the Golgi apparatus, in the matrix of the head cap, and on the head cap membrane in step-4 to step-7 spermatids. The antigen appears to be concentrated at the equatorial segment during late spermiogenesis. Neither the (pro-)acrosomic granule nor the surrounding membrane are required in this pathway. This pathway can be termed the ‘Golgi-head cap tract'. Springer-Verlag, 1992 Acrosome nationallicence Equatorial segment of spermatozoa nationallicence Transport pathway nationallicence Spermiogenesis nationallicence Monoclonal antibody nationallicence Immuno-electron microscopy nationallicence Mouse (BALB/c nationallicence CD-1 nationallicence ICR) nationallicence Rat (Wistar) nationallicence Golden hamster-Human nationallicence Toshimori K. Department of Anatomy, Miyazaki Medical College, Kihara 5200, Kiyotake, 889-16, Miyazaki, Japan aut Tanii I. Department of Anatomy, Miyazaki Medical College, Kihara 5200, Kiyotake, 889-16, Miyazaki, Japan aut Araki S. Department of Anatomy, Miyazaki Medical College, Kihara 5200, Kiyotake, 889-16, Miyazaki, Japan aut Oura C. Department of Anatomy, Miyazaki Medical College, Kihara 5200, Kiyotake, 889-16, Miyazaki, Japan aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 459-468 0302-766X 270:3<459 1992 270 441 https://doi.org/10.1007/BF00645047 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645047 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toshimori K. Department of Anatomy, Miyazaki Medical College, Kihara 5200, Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 700 1- Tanii I. Department of Anatomy, Miyazaki Medical College, Kihara 5200, Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 700 1- Araki S. Department of Anatomy, Miyazaki Medical College, Kihara 5200, Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 700 1- Oura C. Department of Anatomy, Miyazaki Medical College, Kihara 5200, Kiyotake, 889-16, Miyazaki, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 459-468 0302-766X 270:3<459 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer