caa a22 4500 469083328 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645042 doi (NATIONALLICENCE)springer-10.1007/BF00645042 Proliferative and functional stages of rat ameloblast differentiation as revealed by combined immunocytochemistry against enamel matrix proteins and bromodeoxyuridine [Elektronische Daten] [Andrea Casasco, Alberto Calligaro, Marco Casasco] Summary: A double-staining immunocytochemical technique was used for the simultaneous detection, at the light- and electron-microscopical level, of proliferating bromodeoxyuridine (BrdU)-labelled cells and enamel protein (EP)-producing cells in the inner enamel epithelium (IEE) of rat tooth germ. BrdU-positive cells were found in the region of the IEE close to the cervical loop and never displayed EP-like immunoreactivity. BrdU-immunoreactivity was confined to the nucleus of replicating cells. In contrast, epithelial cells displaying EP-like immunoreactivity were found in the region of the forming dental cusp and were consistently BrdU-negative. EP-like immunoreactivity was detectable in the cytoplasmic compartments involved in the exocrine secretion pathway and in the extra-cellular matrix close to EP-immunoreactive cells. These data support the view that withdrawal from the cell cycle in the IEE is a temporal prerequisite for acquiring the functional competence of secreting EP. Moreover, cycling cells and secretory cells in the IEE constitute two separate compartments that are spatially defined, and that exhibit clear-cut staining patterns with respect to BrdU- and EP-immunoreactivity, respectively. We thus propose that BrdU-incorporation and EP-production may be used as specific markers of the differentiation of the IIE cells in studies of the possible role of growth factors, their receptors and oncoproteins in this tissue. Springer-Verlag, 1992 Ameloblasts nationallicence Differentiation nationallicence Proliferation nationallicence Bromodeoxyuridine nationallicence Enamel matrix proteins nationallicence Immunocytochemistry nationallicence Rat (Wistar) nationallicence Casasco Andrea Institute of Histology and Embryology, University of Pavia, Via Forlanini, 10, I-27100, Pavia, Italy aut Calligaro Alberto Institute of Histology and Embryology, University of Pavia, Via Forlanini, 10, I-27100, Pavia, Italy aut Casasco Marco Institute of Histology and Embryology, University of Pavia, Via Forlanini, 10, I-27100, Pavia, Italy aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 415-423 0302-766X 270:3<415 1992 270 441 https://doi.org/10.1007/BF00645042 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645042 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Casasco Andrea Institute of Histology and Embryology, University of Pavia, Via Forlanini, 10, I-27100, Pavia, Italy aut NATIONALLICENCE 700 1- Calligaro Alberto Institute of Histology and Embryology, University of Pavia, Via Forlanini, 10, I-27100, Pavia, Italy aut NATIONALLICENCE 700 1- Casasco Marco Institute of Histology and Embryology, University of Pavia, Via Forlanini, 10, I-27100, Pavia, Italy aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 415-423 0302-766X 270:3<415 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer