caa a22 4500 469083360 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645044 doi (NATIONALLICENCE)springer-10.1007/BF00645044 Colocalization of neuropeptide Y (NPY)-like and FMRFamide-like immunoreactivities in the brain of the Atlantic salmon ( Salmo salar ) [Elektronische Daten] [Elena Vecino, Peter Ekström] Summary: The colocalization of the peptides neuropeptide Y (NPY) and Phe-Met-Arg-Phe-NH2 (FMRFamide) in the brain of the Atlantic salmon was investigated with double immunofluorescence labeling and peroxidase-antiperoxidase immunocytochemical techniques. Colocalization of NPY-like and FMRE amide-like immunoreactivities was observed in neuronal cell bodies and fibers in four brain regions: in the lateral and commissural nuclei of the area ventralis telencephali, in the nucleus ventromedialis thalami, in the laminar nucleus of the mesencephalic tegmentum, and in a group of small neurons situated among the large catecholaminergic neurons in the isthmal region of the brainstem. All cell bodies in these nuclei were immunoreactive to both NPY and FMRF. We consistently observed larger numbers of FMRF-immunoreactive than NPY-immunoreactive fibers. In the nucleus ventromedialis thalami NPY- and FMRFamide-like immunoreactivities were colocalized in cerebrospinal fluid (CSF)-contacting neurons. NPY-immunoreactive, but not FMRF-immunoreactive, neurons were found in the stratum periventriculare of the optic tectum, and at the ventral border of the nucleus habenularis (adjacent to the nucleus dorsolateralis thalami). Neurons belonging to the nucleus of the nervus terminalis were FMRF-immunoreactive but not NPY-immunoreactive. The differential labeling indicates, as do our cross-absorption experiments, that the NPY and FMRFamide antisera recognize different epitopes. Thus, it is probable that NPY-like and FMRFamide-like substances occur in the same neurons in some brain regions. Springer-Verlag, 1992 Neuropeptides nationallicence Neuropeptide Y nationallicence FMRFamide nationallicence Immunocytochemistry nationallicence Telencephalon nationallicence Thalamus nationallicence Salmo salar (Teleostei) nationallicence Vecino Elena Department of Cell Biology and Pathology, University of Salamanca, Avd. Campo Charro s/n, E-37007, Salamanca, Spain aut Ekström Peter Department of Zoology, University of Lund, Helgonavägen 3, S-223 62, Lund, Sweden aut Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 435-442 0302-766X 270:3<435 1992 270 441 https://doi.org/10.1007/BF00645044 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645044 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vecino Elena Department of Cell Biology and Pathology, University of Salamanca, Avd. Campo Charro s/n, E-37007, Salamanca, Spain aut NATIONALLICENCE 700 1- Ekström Peter Department of Zoology, University of Lund, Helgonavägen 3, S-223 62, Lund, Sweden aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 435-442 0302-766X 270:3<435 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer