caa a22 4500 469083387 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00645064 doi (NATIONALLICENCE)springer-10.1007/BF00645064 Carlberg Mats Department of Zoology, University of Lund, Helgonavägen 2, S-223 6, Lund, Sweden aut Localization of dopamine in the freshwater hydrozoan Hydra attenuata [Elektronische Daten] [Mats Carlberg] Summary: High performance liquid chromatography (HPLC), with electrochemical detection, is an analytical method sensitive enough to permit quantification of dopamine, dihydroxyphenylalanine (DOPA) and 5-S-cysteinyl DOPA in single or hemisected specimens ofHydra attenuata. Dopamine and 5-S-cysteinylDOPA appear to be the quantitatively predominant catechol compounds inH. attenuata, whereas DOPA is present in minor amounts. The presence of DOPA and 5-S-cysteinylDOPA, and the quantitative correlation between dopamine and these compounds in many specimens, suggests that dopamine inH. attenuata, as in higher animals, is formed through decarboxylation of DOPA. Contrary to the dopaminergic nerves in higher animals, DOPA inHydra seems to be oxidized and 5-S-cysteinyl DOPA is formed as a by-product. The oxidation of DOPA indicates that the hydroxylation of tyrosine into DOPA in the tissues ofH. attenuata is mediated by a tyrosinase rather than a tyrosine hydroxylase. Immunocytochemical methods demonstrate a highly variable distribution of dopamine in the tissues of different specimens ofH. attenuata. Dopamine immunoreactivity is confined to ectodermal tissue and can be found in several different cell types including nerve cells, battery cells, nematocytes, epithelial cells and interstitial undifferentiated cells. The large amounts of dopamine found in some specimens ofH. attenuata indicate some biological function, although its sporadic occurrence in neurites makes it less plausible as a generally utilized neurotransmitter in this animal. Springer-Verlag, 1992 Dopamine nationallicence HPLC nationallicence Immunocytochemistry nationallicence Hydra attenuata (Cnidaria) nationallicence Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 601-607 0302-766X 270:3<601 1992 270 441 https://doi.org/10.1007/BF00645064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00645064 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Carlberg Mats Department of Zoology, University of Lund, Helgonavägen 2, S-223 6, Lund, Sweden aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/3(1992-12-01), 601-607 0302-766X 270:3<601 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer