caa a22 4500 469083409 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00328013 doi (NATIONALLICENCE)springer-10.1007/BF00328013 Characterization of macrophage-like cells in the external layers of human small and large intestine [Elektronische Daten] [H. Mikkelsen, J. Rumessen] Summary: In the external layers of human small and large intestine macrophage-like cells were characterized by immunohistochemical, histochemical and electronmicroscopical methods. Using immunohistochemistry and a number of monoclonal antibodies, the presence and distribution of phenotypic subpopulations of macrophages were evaluated. In all locations macrophage-like cells were identified with antibody EBM11, which recognizes CD68 antigen, C3bi which recognizes CD11b, and partly with an antibody which recognizes protein 150,95 (CD11c). Macrophage-like cells in the external muscle layer were HLA-DR-positive (expressing the MHC class-II antigen), in contrast to macrophage-like cells in the subserosa and submucosa. Macrophage-like cells in the external muscle layer were mostly acid phosphatase-negative, and at the electron-microscopic level they were found to have features of macrophages: primary lysosomes, coated vesicles and pits. However, very few secondary lysosomes were present. Birbeck granules were not observed. It is concluded that in the external muscle layer of human small and large intestine numerous macrophages of a special type are present. It is discussed whether this cell type plays a role in gastrointestinal motility and/or has an immunological function. Springer-Verlag, 1992 Macrophages nationallicence Small intestine nationallicence Large intestine nationallicence External muscle layer nationallicence Immunohistochemistry nationallicence Histochemistry nationallicence Electron microscopy nationallicence Man nationallicence Mikkelsen H. Anatomy Department C, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut Rumessen J. Anatomy Department C, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 273-279 0302-766X 270:2<273 1992 270 441 https://doi.org/10.1007/BF00328013 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00328013 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mikkelsen H. Anatomy Department C, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Rumessen J. Anatomy Department C, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 273-279 0302-766X 270:2<273 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer