caa a22 4500 469083425 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00328023 doi (NATIONALLICENCE)springer-10.1007/BF00328023 Immunocytochemical and electron-microscopic study of the elasmobranch nucleus sacci vasculosi [Elektronische Daten] [Pilar Molist, Isabel Rodríguez-Moldes, Ramón Anadón] Summary: The elasmobranch nucleus sacci vasculosi was studied by means of electron microscopy (in the dogfish) and immunocytochemistry (in the dogfish and the skate) by using antibodies against tyrosine hydroxylase, alpha-melanocyte-stimulating hormone, somatostatin, serotonin, and substance P. Ultrastructural study of the dogfish nucleus sacci vasculosi shows the presence of medium-sized cells that possess numerous mitochondria but that have no dense-core vesicles in the cytoplasm or in cell processes. Fibres of the conspicuous tractus sacci vasculosi have a beaded appearance and form conventional synapses with dendrites and cell perikarya of the nucleus sacci vasculosi. The perikarya of this hypothalamic nucleus were not immunoreactive to any of the antibodies tested, and fibres immunopositive to tyrosine hydroxylase, alpha-melanocyte-stimulating hormone, somatostatin, serotonin, and substance P were scarce within this nucleus, in both the dogfish and the skate. Dorsal to the nucleus sacci vasculosi, there are numerous positive neuronal processes in addition to many small neurons that show immunoreactivity to alpha-melanocyte-stimulating hormone, somatostatin and tyrosine hydroxylase. Two types of neuron occur in this dorsal region, displaying dense-core vesicles of either 100-160 nm or 60-100 nm diameter in their cytoplasm; they were identified as peptide-containing and monoamine-containing neurons, respectively. The neuropil of this region has a significantly different ultrastructure from that of the nucleus sacci vasculosi, with many processes containing dense-core vesicles. This group of neurons, located dorsal to the nucleus sacci vasculosi and showing (a) immunoreactivity to neuropeptides or to monoamine-synthesizing enzyme, and (b) cytoplasm with dense-core vesicles, was considered not to be a part of the nucleus sacci vasculosi but rather part of the nucleus tuberculi posterioris. These results support the non-peptidergic and non-aminergic character of the nucleus sacci vasculosi. Springer-Verlag, 1992 Nucleus sacci vasculosi nationallicence Ultrastructure nationallicence Immunocytochemistry nationallicence Hypothalamus nationallicence Tuberculum posterius nationallicence Scyliorhinus caniculus, Raja undulata (Elasmobranchii) nationallicence Molist Pilar Department of Fundamental Biology, University of Vigo, P.O. Box 874, E-36200, Vigo, Spain aut Rodríguez-Moldes Isabel Department of Fundamental Biology, University of Santiago de Compostela, E-15706, Santiago de Compostela, Spain aut Anadón Ramón Department of Fundamental Biology, University of Santiago de Compostela, E-15706, Santiago de Compostela, Spain aut Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 395-404 0302-766X 270:2<395 1992 270 441 https://doi.org/10.1007/BF00328023 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00328023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Molist Pilar Department of Fundamental Biology, University of Vigo, P.O. Box 874, E-36200, Vigo, Spain aut NATIONALLICENCE 700 1- Rodríguez-Moldes Isabel Department of Fundamental Biology, University of Santiago de Compostela, E-15706, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Anadón Ramón Department of Fundamental Biology, University of Santiago de Compostela, E-15706, Santiago de Compostela, Spain aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 395-404 0302-766X 270:2<395 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer