caa a22 4500 469083441 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00328008 doi (NATIONALLICENCE)springer-10.1007/BF00328008 The distribution of pheromone-biosynthesis-activating neuropeptide (PBAN) immunoreactivity in the central nervous system of the corn earworm moth, Helicoverpa zea [Elektronische Daten] [Timothy Kingan, Michael Blackburn, Ashok Raina] Summary: Production of sex pheromone in several species of moths has been shown to be under the control of a neuropeptide termed pheromone-biosynthesis-activating neuropeptide (PBAN). We have produced an antiserum to PBAN from Helicoverpa zea (Lepidoptera: Noctuidae) and used it to investigate the distribution of immunoreactive peptide in the brain-suboesophageal ganglion complex and its associated neurohemal structures, and the segmental ganglia of the ventral nerve cord. Immunocytochemical methods reveal three clusters of cells along the ventral midline in the suboesophageal ganglion (SOG), one cluster each in the presumptive mandibular (4 cells), maxillary (12-14 cells), and labial neuromeres (4 cells). The proximal neurites of these cells are similar in their dorsal and lateral patterns of projection, indicating a serial homology among the three clusters. Members of the mandibular and maxillary clusters have axons projecting into the maxillary nerve, while two additional pairs of axons from the maxillary cluster project into the ventral nerve cord. Members of the labial cluster project to the retrocerebral complex (corpora cardiaca and cephalic aorta) via the nervus corpus cardiaci III (NCC III). The axons projecting into the ventral nerve cord appear to arborize principally in the dorsolateral region of each segmental ganglion; the terminal abdominal ganglion is distinct in containing an additional ventromedial arborization in the posterior third of the ganglion. Quantification of the extractable immunoreactive peptide in the retrocerebral complex by ELISA indicates that PBAN is gradually depleted during the scotophase, then restored to maximal levels in the photophase. Taken together, our findings provide anatomical evidence for both neurohormonal release of PBAN as well as axonal transport via the ventral nerve cord to release sites within the segmental ganglia. Springer-Verlag, 1992 Pheromone-biosynthesis-activating neuropeptide nationallicence Immunocytochemistry nationallicence Helicoverpa zea (Insecta) nationallicence A : aorta nationallicence Br-SOG : brain-suboesophageal ganglion complex nationallicence CC : corpus cardiacum nationallicence PBS : phosphate-buffered saline nationallicence PLI : PBAN-like immunoreactivity nationallicence TAG : terminal abdominal ganglion nationallicence VNC : ventral nerve cord nationallicence Kingan Timothy Insect Neurobiology and Hormone Laboratory, Agricultural Research Service, USDA, Bldg. 306, Rm. 322, 20705, Beltsville, MD, USA aut Blackburn Michael Department of Entomology, University of Maryland, College Park, Maryland, USA aut Raina Ashok Insect Neurobiology and Hormone Laboratory, Agricultural Research Service, USDA, Bldg. 306, Rm. 322, 20705, Beltsville, MD, USA aut Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 229-240 0302-766X 270:2<229 1992 270 441 https://doi.org/10.1007/BF00328008 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00328008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kingan Timothy Insect Neurobiology and Hormone Laboratory, Agricultural Research Service, USDA, Bldg. 306, Rm. 322, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Blackburn Michael Department of Entomology, University of Maryland, College Park, Maryland, USA aut NATIONALLICENCE 700 1- Raina Ashok Insect Neurobiology and Hormone Laboratory, Agricultural Research Service, USDA, Bldg. 306, Rm. 322, 20705, Beltsville, MD, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 229-240 0302-766X 270:2<229 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer