caa a22 4500 46908345X CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00328007 doi (NATIONALLICENCE)springer-10.1007/BF00328007 Anatomical identification of glomeruli in the antennal lobes of the male sphinx moth Manduca sexta [Elektronische Daten] [J. Rospars, J. Hildebrand] Summary: Computer-assisted neuroanatomical methods have been used to demonstrate unique identities of the glomeruli of the antennal lobes (ALs) in males of the sphinx moth Manduca sexta. The glomerular neuropil consists of the male-specific macroglomerular complex, which comprises two closely apposed bulky subunits, and 64±1 "ordinary” glomeruli arrayed in a shell around a central region of coarse neuropil. Computergenerated maps show the exact locations of all glomeruli and adjacent groups of neuronal somata in a constant Cartesian coordinate system, such that these can be accurately identified in any individual. The glomeruli belong to three classes according to the number and type of identification criteria they satisfy. The larger class comprises glomeruli (n=44) identified only in the computer-generated maps on the basis of their relative positions. The other two classes include glomeruli that were also identified in sections, either directly from their proximity to readily identifiable structures and their shape and size (n=10, including the labial-palp-pit-organ (LPO) glomerulus), or indirectly from their positions relative to the former (n=9). Two very small glomeruli were present in only one AL, demonstrating the existence of anomalous glomeruli, whereas another glomerulus had no homologue in both ALs of one individual. The true number of ordinary glomeruli (per male AL) was thus estimated to be 64. The uncertainty in delineating some glomeruli might affect this number without implying modification of the homologies proposed. The locations of tracts and cell groups, both within and near the AL, are also invariant with respect to glomeruli, as shown in the computer maps. The methods employed are general and might be useful to researchers in related fields. The results obtained call for more attention to the precise geometry of neural structures. Springer-Verlag, 1992 Insect nervous system nationallicence Antennal lobe nationallicence Olfactory system nationallicence Manduca sexta (Insecta) nationallicence Rospars J. Laboratoire de Biométrie, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78000, Versailles, France aut Hildebrand J. ARL Division of Neurobiology, University of Arizona, Tucson, Arizona, USA aut Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 205-227 0302-766X 270:2<205 1992 270 441 https://doi.org/10.1007/BF00328007 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00328007 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rospars J. Laboratoire de Biométrie, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78000, Versailles, France aut NATIONALLICENCE 700 1- Hildebrand J. ARL Division of Neurobiology, University of Arizona, Tucson, Arizona, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 205-227 0302-766X 270:2<205 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer