caa a22 4500 469083522 CHVBK 20180323132957.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00328018 doi (NATIONALLICENCE)springer-10.1007/BF00328018 Ontogeny of the endocrine pancreas in sea bass ( Dicentrarchus labrax ) [Elektronische Daten] An immunocytochemical study [M. García Hernández, B. Agulleiro] Summary: The development of the endocrine pancreas of the teleost sea bass (Dicentrarchus labrax, L.) was examined from hatching to 61 days, using the peroxidase-antiperoxidase technique for light microscopy. Mammalian and bonito insulin (mI and bI)-, salmo somatostatin-25 (SST-25)-, somatostatin-14 (SST-14a and b)-, glucagon-, bovine pancreatic polypeptide (PP)-, peptide tyrosine-tyrosine (PYY)- and salmo neuropeptide Y (NPY)-like immunoreactivity was demonstrated. Four ontogenetic stages were established according to the organization and immunostaining of the endocrine cells. One cell strand or primordial cord showing mI/bI- and SST-25/SST-14a-like immunoreactivity was first found at hatching in the dorsal epithelium of the anterior zone of the midgut (stage 1). One primitive islet, comprising outer SST-25/SST-14a- and inner mI/bI- and SST-14a/ SST-14b-immunoreactive cells, was found in 2- to 5-day-old larvae (stage 2). One single islet, in which glucagon-immunoreactive cells appear in the periphery, was found in larvae from 9 to 20 days after hatching (stage 3). One big islet containing, in addition, PP-immunoreactive cells in the outer region and slender cell processes which showed PYY-like immunoreactivity, was found from 25 to 61 days after hatching. During this period, primordial islets, composed of SST-25- and bI-immunoreactive cells, and clustered or isolated pancreatic endocrine cells, close to the pancreatic duct, as well as small and intermediate islets (secondary islets), in which glucagon, PP, PYY and NPY seem to be co-localized, were progressively found (stage 4). The origin of the endocrine pancreas of sea bass, and the ontogenetic and phylogenetic significance, are discussed. Springer-Verlag, 1992 Endocrine pancreas nationallicence Ontogeny nationallicence Regulatory peptides nationallicence Immunocytochemistry nationallicence Dicentrarchus labrax (Teleostei) nationallicence García Hernández M. Department of Cell Biology, Faculty of Biology, University of Murcia, E-30100, Murcia, Spain aut Agulleiro B. Department of Cell Biology, Faculty of Biology, University of Murcia, E-30100, Murcia, Spain aut Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 339-352 0302-766X 270:2<339 1992 270 441 https://doi.org/10.1007/BF00328018 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00328018 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- García Hernández M. Department of Cell Biology, Faculty of Biology, University of Murcia, E-30100, Murcia, Spain aut NATIONALLICENCE 700 1- Agulleiro B. Department of Cell Biology, Faculty of Biology, University of Murcia, E-30100, Murcia, Spain aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 339-352 0302-766X 270:2<339 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 469083522 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer