caa a22 4500 469083557 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00328011 doi (NATIONALLICENCE)springer-10.1007/BF00328011 Localization of xenopsin and xenopsin precursor fragment immunoreactivities in the skin and gastrointestinal tract of Xenopus laevis [Elektronische Daten] [Kirsten Sadler, Charles Bevins, Jane Kaltenbach] Summary: Xenopsin (Xp) and xenopsin precursor fragment (XPF) are bioactive peptides derived from a single precursor molecule; both were isolated previously from extracts of Xenopus laevis skin. The present immunohistochemical study was undertaken to determine the specific cellular localization of these two peptides in the skin and also in the gastrointestinal tract of adult Xenopus. We report here that Xp-like and XPF-like immuno-reactivities co-exist in the granular glands of the skin and specific granular cells in the lower esophagus and stomach. However, only Xp-like immunoreactivity, not XPF-like immunoreactivity, was detected in tall, thin cells of the duodenum and in club-shaped cells of the large intestine. The immunochemical co-localization of the two peptides in specific cells of the skin, lower esophagus and stomach suggests that the same gene is expressed in each of these cells, and that the precursor molecule undergoes similar post-translational processing. In contrast, the observation that certain cells of the duodenum and large intestine display only one peptide immunoreactivity suggests an alternative phenomenon, possibly involving selective peptide accumulation or expression of a different gene. Springer-Verlag, 1992 Xenopsin nationallicence Xenopsin precursor fragment nationallicence Immunohistochemistry nationallicence Skin nationallicence Gastrointestinal tract nationallicence Xenopus laevis (Anura) nationallicence Sadler Kirsten Department of Biological Sciences, Mount Holyoke College, 01075, South Hadley, MA, USA aut Bevins Charles Division of Human Genetics and Molecular Biology, The Children's Hospital of Philadelphia and Department of Pediatrics, University of Pennsylvania School of Medicine, 19104, Philadelphia, PA, USA aut Kaltenbach Jane Department of Biological Sciences, Mount Holyoke College, 01075, South Hadley, MA, USA aut Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 257-263 0302-766X 270:2<257 1992 270 441 https://doi.org/10.1007/BF00328011 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00328011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sadler Kirsten Department of Biological Sciences, Mount Holyoke College, 01075, South Hadley, MA, USA aut NATIONALLICENCE 700 1- Bevins Charles Division of Human Genetics and Molecular Biology, The Children's Hospital of Philadelphia and Department of Pediatrics, University of Pennsylvania School of Medicine, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Kaltenbach Jane Department of Biological Sciences, Mount Holyoke College, 01075, South Hadley, MA, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 270/2(1992-11-01), 257-263 0302-766X 270:2<257 1992 270 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer