caa a22 4500 469083611 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384721 doi (NATIONALLICENCE)springer-10.1007/BF00384721 Neuronal influence on B and H human blood-group antigen expression in rat cochlear cultures [Elektronische Daten] [Pablo Gil-Loyzaga, Manuel Remezal, Rafael Oriol] Summary: The presence of B and H human blood-group antigens was analyzed by immunocytochemistry in rat cochleas developing either in vivo or in vitro. Developing animals, on embryonic day (E) 18 and postnatal day (P) 3, were used for in vivo studies. For in vitro studies, cochleas were removed at E18 and placed for 3 or 8 days in organotypic culture either directly or after partial spiral ganglion removal. Results from epithelial regions from cochleas developing in vivo were similar to those observed in corresponding areas of direct organotypic cultures where the innervation from spiral ganglion neurons was present. Antibodies to human blood group antigens, anti B and anti AB, selectively labeled hair cells. The intensity of labeling was weak at E18, but increased at P3 in vivo or after 3-8 days in organotypic culture. Anti H antibodies showed weak labeling of the apical surface of hair cells and other epithelial cells at E18; this labeling also increased at P3 or after 3-8 days in culture. In contrast, the non-innervated regions from organotypic cultures, where ganglia were partially removed, exhibited an epithelial disorganization and no hair cell labeling with any of the antibodies studied. The present findings suggest that human blood-group antigen expression on developing cochlear hair cells of rats may be related to afferent nerve fiber influence. Springer-Verlag, 1992 Trophic influence nationallicence Cell differentiation nationallicence Blood-group antigens nationallicence Cochlea nationallicence Hair cells nationallicence Organ culture nationallicence Rat (Sprague-Dawley) nationallicence Gil-Loyzaga Pablo Departmento Ciencias Morfologicas, Facultad de Medicina, Universidad Complutense, Apartado de Correos 60075, E-28080, Madrid, Spain aut Remezal Manuel Departmento Ciencias Morfologicas, Facultad de Medicina, Universidad Complutense, Apartado de Correos 60075, E-28080, Madrid, Spain aut Oriol Rafael CNRS, Biochimie, Faculté de Pharmacie, Université Paris-Sud, Chatenay-Malabry, France aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 13-20 0302-766X 269:1<13 1992 269 441 https://doi.org/10.1007/BF00384721 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384721 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gil-Loyzaga Pablo Departmento Ciencias Morfologicas, Facultad de Medicina, Universidad Complutense, Apartado de Correos 60075, E-28080, Madrid, Spain aut NATIONALLICENCE 700 1- Remezal Manuel Departmento Ciencias Morfologicas, Facultad de Medicina, Universidad Complutense, Apartado de Correos 60075, E-28080, Madrid, Spain aut NATIONALLICENCE 700 1- Oriol Rafael CNRS, Biochimie, Faculté de Pharmacie, Université Paris-Sud, Chatenay-Malabry, France aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 13-20 0302-766X 269:1<13 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer