caa a22 4500 46908362X CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384737 doi (NATIONALLICENCE)springer-10.1007/BF00384737 Cellular immunity in an annelid ( Nereis diversicolor , Polychaeta): production of melanin by a subpopulation of granulocytes [Elektronische Daten] [Eliane Porchet-Henneré, Guy Vernet] Summary: We attempted to identify the nature and origin of the pigment produced by the marine worm Nereis diversicolor in order to isolate, in inert brown capsules, foreign objects introduced into its body cavity. This brown pigment, characterized by cytochemical techniques, could be a melanin. The activity of the enzyme phenoloxidase responsible for melanin biosynthesis was detected by enzyme cytochemistry techniques in vacuoles and the Golgi apparatus of coelomocytes activated by the presence of foreign bodies. Morphological techniques combined with a monoclonal immunological probe enabled us to establish that the "G2” granulocytes contain both the precursor of the pigment in dense bodies and the capacity for phenoloxidase synthesis when activated to encapsulate foreign bodies. The "G2” granulocyte may therefore be compared to a melanocyte in which melanin is not stored as in mammals, but immediately extruded following synthesis in the form of a thick fluid. Springer-Verlag, 1992 Invertebrate immunity nationallicence Coelomocytes nationallicence Encapsulation nationallicence Melanin nationallicence Cytochemistry nationallicence Immunohistochemistry nationallicence Nereis diversicolor (Annelida) nationallicence ACTH : adrenocorticotropic hormone nationallicence Dopa L-3,4 : dihydroxyphenylalanine nationallicence FITC : fluorescein isothiocyanate nationallicence G 1, G 2, G 3 : granulocyte of types 1, 2, 3 nationallicence MSH : melanocyte-stimulating hormone nationallicence proPo : prophenoloxidase nationallicence Porchet-Henneré Eliane URA 148 CNRS, Laboratoire de Biologie animale, Université des Sciences et Technologies de Lille, F-59655, Villeneuve d'Ascq Cedex, France aut Vernet Guy UR 4R, Laboratoire de Zoologie et des Sciences de l'environnement, Faculté des Sciences, Université de Reims Champagne Ardennes, "Moulin de la Housse”, B.P. 347, F-51062, Reims Cedex, France aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 167-174 0302-766X 269:1<167 1992 269 441 https://doi.org/10.1007/BF00384737 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384737 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Porchet-Henneré Eliane URA 148 CNRS, Laboratoire de Biologie animale, Université des Sciences et Technologies de Lille, F-59655, Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Vernet Guy UR 4R, Laboratoire de Zoologie et des Sciences de l'environnement, Faculté des Sciences, Université de Reims Champagne Ardennes, "Moulin de la Housse”, B.P. 347, F-51062, Reims Cedex, France aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 167-174 0302-766X 269:1<167 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer