caa a22 4500 469083638 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384730 doi (NATIONALLICENCE)springer-10.1007/BF00384730 Immunocytochemical localization of muscarinic acetylcholine receptors in the rat endocrine pancreas [Elektronische Daten] [E. Van der Zee, B. Buwalda, J. Strubbe, A. Strosberg, P. Luiten] Summary: Immunocytochemical application of the antimuscarinic acetylcholine receptor antibody M35 to pancreas tissue revealed the target areas for the parasympathetic nervous system. Immunoreactivity in the endocrine pancreas was much higher than that in the exocrine part. Moreover, the endocrine cells at the periphery of the islets of Langerhans displayed the highest level of immunoreactivity. Based on these findings in the mantle of the islets, two types of islets have been distinguished: type-I islets with intensely stained mantle cells, and type-II islets with a much lower concentration of these cells. On average, type-I islets were larger (244.8 μm±6.1 SEM) than type-II islets (121.5 μm±3.8 SEM). M35-immunoreactivity was present on the majority of D cells, which were characterized by their immunoreactivity to somatostatin [of 446 D cells 356 (79.8%) were M35-immunopositive]. However, only a small proportion of the intensely stained mantle cells belonged to the D cell population. Therefore, it is concluded that the majority of the intensely stained mantle cells represent glucagon-secreting A and/or pancreatic polypeptide-secreting F cells. The intensity of M35-immunoreactivity at the periphery and central core of the islets paralleled the density of cholinergic innervation, suggesting a positive correlation between the intensity of cholinergic transmission and the number of muscarinic acetylcholine receptors at the target structures. The present study further revealed some striking parallels for the muscarinic acetylcholine receptor characteristics between the (endocrine) pancreas and the central nervous system. Springer-Verlag, 1992 Pancreas, endocrine nationallicence Immunocytochemistry nationallicence Muscarinic acetylcholine receptor nationallicence Acetylcholine nationallicence Somatostatin nationallicence Rat (Wistar) nationallicence Van der Zee E. Department of Animal Physiology, University of Groningen, P.O. Box 14, NL-9750 AA, Haren, The Netherlands aut Buwalda B. Department of Animal Physiology, University of Groningen, P.O. Box 14, NL-9750 AA, Haren, The Netherlands aut Strubbe J. Department of Animal Physiology, University of Groningen, P.O. Box 14, NL-9750 AA, Haren, The Netherlands aut Strosberg A. Laboratoire d'Immunopharmacologie Moléculaire, Institut Cochin de Génétique Moléculaire, 22, Rue Méchain, F-75014, Paris, France aut Luiten P. Department of Animal Physiology, University of Groningen, P.O. Box 14, NL-9750 AA, Haren, The Netherlands aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 99-106 0302-766X 269:1<99 1992 269 441 https://doi.org/10.1007/BF00384730 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384730 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van der Zee E. Department of Animal Physiology, University of Groningen, P.O. Box 14, NL-9750 AA, Haren, The Netherlands aut NATIONALLICENCE 700 1- Buwalda B. Department of Animal Physiology, University of Groningen, P.O. Box 14, NL-9750 AA, Haren, The Netherlands aut NATIONALLICENCE 700 1- Strubbe J. Department of Animal Physiology, University of Groningen, P.O. Box 14, NL-9750 AA, Haren, The Netherlands aut NATIONALLICENCE 700 1- Strosberg A. Laboratoire d'Immunopharmacologie Moléculaire, Institut Cochin de Génétique Moléculaire, 22, Rue Méchain, F-75014, Paris, France aut NATIONALLICENCE 700 1- Luiten P. Department of Animal Physiology, University of Groningen, P.O. Box 14, NL-9750 AA, Haren, The Netherlands aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 99-106 0302-766X 269:1<99 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer