caa a22 4500 469083646 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384728 doi (NATIONALLICENCE)springer-10.1007/BF00384728 Kusakabe Tatsumi Department of Anatomy, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut Ontogeny of substance P-, CGRP-, and VIP-containing nerve fibers in the amphibian carotid labyrinth of the bullfrog, Rana catesbeiana [Elektronische Daten] An immunohistochemical study [Tatsumi Kusakabe] Summary: The ontogeny of substance P, CGRP (calcitonin gene-related peptide), and VIP (vasoactive intestinal polypeptide) containing nerve fibers in the carotid labyrinth of the bullfrog, Rana catesbeiana, was examined by the peroxidase-antiperoxidase method. The time of appearance of these three peptides was different for each. First, CGRP fibers appeared in the wall of the carotid arch and external carotid arteries, and in a thin septum between these two arteries at an early stage of larval development (stage III). At stage V, substance P immunoreactive fibers appeared, and VIP fibers were detected at the early metamorphic stage (stage XXII). Up to the completion of metamorphosis, the number of these fibers remained low. From 1 to 5 weeks after metamorphosis, substance P, CGRP, and VIP fibers increased in number to varying degrees. By 8 weeks after metamorphosis, the distribution and abundance of these fibers closely resembled those of the adults. Some CGRP and VIP immunoreactive glomus cells were found at the stages immediately before and after the completion of metamorphosis. These findings suggest that substance P, CGRP, and VIP fibers during larval development and metamorphosis may be nonfunctional, and start to participate in vascular regulation only after metamorphosis. The transient CGRP and VIP in some glomus cells may be important for the development of the labyrinth, or may take part in vascular regulation through the close apposition of the glomus and smooth muscle cells (g-s connection). Springer-Verlag, 1992 Carotid labyrinth nationallicence Ontogeny nationallicence Substance P nationallicence CGRP nationallicence VIP nationallicence Immunohistochemistry nationallicence Rana catesbeiana (Anura) nationallicence Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 79-85 0302-766X 269:1<79 1992 269 441 https://doi.org/10.1007/BF00384728 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384728 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Kusakabe Tatsumi Department of Anatomy, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, 236, Yokohama, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 79-85 0302-766X 269:1<79 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer