caa a22 4500 469083654 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384739 doi (NATIONALLICENCE)springer-10.1007/BF00384739 Differential expression of four genes encoding molluscan insulin-related peptides in the central nervous system of the pond snail Lymnaea stagnalis [Elektronische Daten] [I. Meester, M. Ramkema, J. van Minnen, H. Boer] Summary: In the pond snail Lymnaea stagnalis, the growth regulating system consists of (1) about 200 neuroendocrine light green cells, located in four clusters in the cerebral ganglia, and (2) the paired canopy cells, located in the lateral lobes. These cells express genes encoding the molluscan insulin-related peptides (MIPs). Six MIP genes have previously been identified. Four of these (I, II, III and V) are expressed in the light green cells and the canopy cells. The MIP-VI gene is a pseudogene. In the present in situ hybridization study, using oligonucleotide probes specific to the transcripts of MIP-I,-II,-III,-IV, and-V, no signal was obtained with the MIP-IV probe, indicating that gene IV is also a pseudogene. With the other four probes, two types of light green cells were distinguished. Type-A cells express all four MIP genes, whereas type-B cells do not (or only faintly) express the MIP-I gene. Gene III is relatively strongly expressed in type-B cells. Genes II and V are moderately expressed in both cell types. Type-A cells are mainly located in the periphery of the clusters, whereas type-B cells are present in the center. The canopy cell resembles type-A light green cells. The expression levels of the MIP-II and MIP-V genes are low in the canopy cell. The expression pattern of the MIP genes correlates with the staining pattern of the anti-MIP-C antibody, which has been raised to a synthetic C-fragment shared by MIP-I,-II and-V. Type-A cells stain more intensely with the antibody than type-B cells. Springer-Verlag, 1992 Molluscan insulin-related peptide nationallicence Neuropeptide nationallicence Light green cells nationallicence Differential gene expression nationallicence Immunocytochemistry nationallicence In situ hybridization nationallicence Lymnaea stagnalis (Mollusca) nationallicence Meester I. Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut Ramkema M. Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut van Minnen J. Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut Boer H. Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 183-188 0302-766X 269:1<183 1992 269 441 https://doi.org/10.1007/BF00384739 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384739 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meester I. Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Ramkema M. Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- van Minnen J. Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Boer H. Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 183-188 0302-766X 269:1<183 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer