caa a22 4500 469083697 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384720 doi (NATIONALLICENCE)springer-10.1007/BF00384720 Regeneration of autotransplanted splenic tissue at different implantation sites [Elektronische Daten] [Andrea Liaunigg, Christine Kastberger, Wolfgang Leitner, Marion Kurz, Elke Bergmann, Manfred Seifriedsberger, Daniela Weinlich, Wolfgang Pimpl, Joseph Thalhamer] Summary: Inbred animals (Lewis rats) were used to investigate the regeneration of autologously implanted splenic tissue at intra-omental and subcutaneous sites. Quantitative immunohistology with monoclonal antibodies against lymphocytes and macrophages was performed to analyse the cell density of red pulp (RP), periarteriolar lymphoid sheath (PALS), marginal zone (MZ) and follicle, 7-180 days after transplantation. Antigenic, allogeneic and mitogenic stimulation and Northern blotting were also performed. Transplant groups differed from spleen only in the reduced size of PALS; however, quantitative analysis demonstrated subtle differences between spleen and transplants. The cell density of B-cells and ED-1+ macrophages was reduced in the RP, Tsupp/cyt-cells were decreased and B-cells increased in PALS, and B-cells and Thelper-cells reduced in the MZ. No differences could be detected between the transplant groups. Flow-cytometric analysis of cell suspensions from spleen and transplants revealed a reduction of T-cells (OX-19+), MHC-I and transferrin-receptor-bearing cells in both transplant groups, and a decrease in the number of Thelper-cells and ED-3+ macrophages in subcutaneous transplants. Both transplant groups were defective regarding the allogeneic and pokeweed mitogen response. Aberration of the lipopolysaccharide response was restricted to subcutaneous transplants, which additionally showed abnormal expression of interferon-gamma, interleukin-5 and interleukin-6 mRNA. Thus, subtle alterations of the newly developed microenvironment and/or lymphocyte-homing may influence the regeneration of splenic tissue; the implantation site may represent an important parameter in functional reorganisation. Springer-Verlag, 1992 Spleen nationallicence Transplantation nationallicence Immunohistochemistry nationallicence Lymphocytes nationallicence Macrophages nationallicence Stimulation nationallicence Cytokines nationallicence Rat (Lewis) nationallicence Rat (Wistar) nationallicence Liaunigg Andrea Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut Kastberger Christine Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut Leitner Wolfgang Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut Kurz Marion Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut Bergmann Elke Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut Seifriedsberger Manfred Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut Weinlich Daniela Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut Pimpl Wolfgang Ludwig Boltzmann Institut für Experimentelle und Gastroenterologische Chirurgie, Müllner Hauptstrasse 48, A-5020, Salzburg, Austria aut Thalhamer Joseph Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 1-11 0302-766X 269:1<1 1992 269 441 https://doi.org/10.1007/BF00384720 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384720 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liaunigg Andrea Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut NATIONALLICENCE 700 1- Kastberger Christine Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut NATIONALLICENCE 700 1- Leitner Wolfgang Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut NATIONALLICENCE 700 1- Kurz Marion Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut NATIONALLICENCE 700 1- Bergmann Elke Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut NATIONALLICENCE 700 1- Seifriedsberger Manfred Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut NATIONALLICENCE 700 1- Weinlich Daniela Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut NATIONALLICENCE 700 1- Pimpl Wolfgang Ludwig Boltzmann Institut für Experimentelle und Gastroenterologische Chirurgie, Müllner Hauptstrasse 48, A-5020, Salzburg, Austria aut NATIONALLICENCE 700 1- Thalhamer Joseph Abteilung für Biochemie, Institut für Allgemeine Biologie, Biochemie und Biophysik, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 1-11 0302-766X 269:1<1 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer