caa a22 4500 469083719 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384735 doi (NATIONALLICENCE)springer-10.1007/BF00384735 Immunohistochemical localisation of natriuretic peptides in the heart and brain of the gulf toadfish Opsanus beta [Elektronische Daten] [John Donald, David Evans] Summary: The distribution of natriuretic peptide immunoreactivity was determined in the heart and brain of the gulf toadfish Opsanus beta using the avidin-biotin peroxidase technique. Four antisera were used: the first raised against porcine brain natriuretic peptide which cross-reacts with atrial natriuretic and C-type natriuretic peptides (termed natriuretic peptide-like immunoreactivity); the second raised against porcine brain natriuretic peptide which cross-reacts with C-type natriuretic peptide but not with atrial natriuretic peptide (termed porcine brain natriuretic peptide-like immunoreactivity); the third raised against rat atrial natriuretic peptide; and the fourth raised against eel atrial natriuretic peptide. Natriuretic peptide- and porcine brain natriuretic peptide-like immunoreactivity was observed in all cardiac muscle cells of the atrium. In the ventricle, natriuretic peptide-like immunoreactivity was found in all cardiac muscle cells, however porcine brain natriuretic peptidelike immunoreactivity was confined to muscle cells adjacent to the epicardium. There was no discernible difference in the distribution of natriuretic peptide-like immunoreactivity and porcine brain natriuretic peptide-like immunoreactivity in the brain. Immunoreactive perikarya were observed only in the preoptic region of the diencephalon, and many immunoreactive fibres were found in the telencephalon, preoptic area, and rostral hypothalamus, lateral to the thalamic region. There was no immunoreactivity in any region of the hypophysis. A pair of distinct immunoreactive fibre tracts ran caudally from the preoptic area to the thalamic region, from which fibres extended to the posterior commissure, area praetectalis, dorsolateral regions of the midbrain tegmentum, and tectum. Many immunoreactive fibres were present in the rostral regions of the inferior lobes of the hypothalamus and in the dorsolateral and ventrolateral aspects of the rhombencephalon. No immunoreactivity was observed in the heart and brain using rat atrial natriuretic and eel natriuretic peptide antisera. Although the chemical structure of natriuretic peptides in the heart and brain of toadfish is unknown, these observations show that a component of the natriuretic peptide complement is similar to porcine brain natriuretic and/or porcine C-type natriuretic peptides. The presence of natriuretic peptides in the brain suggests that they could be important neuromodulators and/or neurotransmitters. Springer-Verlag, 1992 Atrial natriuretic peptide nationallicence Brain natriuretic peptide nationallicence C-type natriuretic peptide nationallicence Heart nationallicence Brain vertebrate nationallicence Immunohistochemistry nationallicence Opsanus beta (Teleostei) nationallicence Donald John Department of Zoology, University of Florida, 32611, Gainesville, FL, USA aut Evans David Department of Zoology, University of Florida, 32611, Gainesville, FL, USA aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 151-158 0302-766X 269:1<151 1992 269 441 https://doi.org/10.1007/BF00384735 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384735 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donald John Department of Zoology, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Evans David Department of Zoology, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 151-158 0302-766X 269:1<151 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer