caa a22 4500 469083735 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384729 doi (NATIONALLICENCE)springer-10.1007/BF00384729 Projections and pathways of submucous neurons to the mucosa of the guinea-pig small intestine [Elektronische Daten] [Z. Song, S. Brookes, P. Steele, M. Costa] Summary: Double-labelling immunohistochemistry and retrograde transport of the carbocyanine dye, DiI, were used to establish the pathways of submucous neurons to the mucosa of the guinea-pig small intestine. Following the application of DiI to a villus, DiI-labelled nerve cell bodies were found in the submucous plexus up to 8.3 mm circumferentially and 3.8 mm longitudinally. The size of each of the four characterised classes of submucous neurons was determined and their distributions and projections mapped. Cells characterised by vasoactive intestinal polypeptide immunoreactivity accounted for 52% of DiI-labelled cells and had the longest projections. Cells characterised by neuropeptide Y (19%) or by calretinin immunoreactivity (13% of all DiI-labelled neurons) had relatively short projections and cells with substance P immunoreactivity (20%) had intermediate lengths of projection. When DiI was applied directly to the submucous plexus, filled neurons of all classes had significantly shorter projections, indicating that they must run for considerable distances in other pathways to the mucosa, probably via the non-ganglionated plexus. On average, each villus is innervated by at least 70 submucous neurons. From quantitative estimates there are 9 submucous neurons per villus. Thus, each submucous neuron is likely to supply about 8 villi. This demonstrates a high degree of convergence and divergence in the innervation of the mucosa. Springer-Verlag, 1992 Submucous plexus nationallicence Mucosa nationallicence Muscularis mucosae nationallicence Intestine, small nationallicence Retrograde transport nationallicence Dil nationallicence Guinea-pig nationallicence Song Z. Department of Physiology, Centre for Neuroscience, The Flinders University of South Australia, GPO Box 2100, 5001, Adelaide, Australia aut Brookes S. Department of Physiology, Centre for Neuroscience, The Flinders University of South Australia, GPO Box 2100, 5001, Adelaide, Australia aut Steele P. Department of Physiology, Centre for Neuroscience, The Flinders University of South Australia, GPO Box 2100, 5001, Adelaide, Australia aut Costa M. Department of Physiology, Centre for Neuroscience, The Flinders University of South Australia, GPO Box 2100, 5001, Adelaide, Australia aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 87-98 0302-766X 269:1<87 1992 269 441 https://doi.org/10.1007/BF00384729 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384729 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Z. Department of Physiology, Centre for Neuroscience, The Flinders University of South Australia, GPO Box 2100, 5001, Adelaide, Australia aut NATIONALLICENCE 700 1- Brookes S. Department of Physiology, Centre for Neuroscience, The Flinders University of South Australia, GPO Box 2100, 5001, Adelaide, Australia aut NATIONALLICENCE 700 1- Steele P. Department of Physiology, Centre for Neuroscience, The Flinders University of South Australia, GPO Box 2100, 5001, Adelaide, Australia aut NATIONALLICENCE 700 1- Costa M. Department of Physiology, Centre for Neuroscience, The Flinders University of South Australia, GPO Box 2100, 5001, Adelaide, Australia aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 87-98 0302-766X 269:1<87 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer