caa a22 4500 469083743 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384726 doi (NATIONALLICENCE)springer-10.1007/BF00384726 Dye coupling in the corneal endothelium: effects of ouabain and extracellular calcium removal [Elektronische Daten] [Mitchell Watsky, James Rae] Summary: The effects of ouabain and extracellular calcium removal on gap junctional coupling of isolated rabbit corneal endothelium was examined using a modified dye-spread technique. This technique is a modification of a microelectrode procedure that now utilizes patch electrodes connected to a current-clamp circuit for dye iontophoresis and a shuttering system in the excitation light path to reduce phototoxic effects in the monolayer. It was found that a significant degree of junctional uncoupling occurred after 45 min of exposure to ouabain, quantified as a reduction in the effective diffusion coefficient of injected Lucifer yellow CH: 1.74×10-7 cm2/s (control) versus 0.43×10-7 cm2/s (ouabain-treated). It was also determined that no gap junctional uncoupling occurs after extended exposure (up to 3.5 h) to a calcium-free extracellular environment. Springer-Verlag, 1992 Cornea nationallicence Endothelium nationallicence Gap junction nationallicence Ouabain nationallicence Calcium-free solution nationallicence Phototoxicity nationallicence Lucifer yellow nationallicence Rabbit nationallicence Watsky Mitchell Department of Physiology and Biophysics, Mayo Foundation, 55905, Rochester, MN, USA aut Rae James Department of Physiology and Biophysics, Mayo Foundation, 55905, Rochester, MN, USA aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 57-63 0302-766X 269:1<57 1992 269 441 https://doi.org/10.1007/BF00384726 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384726 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Watsky Mitchell Department of Physiology and Biophysics, Mayo Foundation, 55905, Rochester, MN, USA aut NATIONALLICENCE 700 1- Rae James Department of Physiology and Biophysics, Mayo Foundation, 55905, Rochester, MN, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 57-63 0302-766X 269:1<57 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer