caa a22 4500 469083751 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384724 doi (NATIONALLICENCE)springer-10.1007/BF00384724 Morphogenesis of the photoreceptor outer segment during postnatal development in the mouse (BALB/c) retina [Elektronische Daten] [Shuichi Obata, Jiro Usukura] Summary: Disc formation of rod photoreceptor cells in developing BALB/c mice retinas was studied by rapid freeze, freeze-substitution, freeze-etching, immunocytochemistry, and myosin S-1 decoration methods. Freeze-substituted photoreceptor cells contained variously shaped vesicles in the apical swelling of the connecting cilium or the base of the outer segment during postnatal development. Rapid freezing successfully arrested pinocytosis; the fusion of small vesicles to give large ones, and the compression of certain vesicles (0.3-0.6 μm) appears to lead gradually to the formation of the so-called discs. We therefore propose that membranous discs are formed by the fusion of small pinocytotic vesicles and their subsequent compression. Discs formed in this way were partially stacked, but were ordered at random during the early developmental stages. During development, a partial stack of discs was progressively rearranged to a regular form as seen in mature outer segments. Cytoskeletal actin was expected to be involved in the disc formation; it was demonstrated in the distal axoneme of the connecting cilium during development and showed no change in its distribution. However, the polarity of the actin filaments, as revealed by myosin S-1 decoration in early developmental stages, was much more variable than in the adult. Barbed ends of actin filaments were associated with the plasma membrane or the membrane of vesicles. We also found actin filaments coiled up helically on ciliary microtubules. Springer-Verlag, 1992 Retina nationallicence Photoreceptor cells nationallicence Outer segment nationallicence Disc membrane nationallicence Morphogenesis nationallicence Rapid freezing nationallicence Actin immunocytochemistry nationallicence Mouse (BALB/c) nationallicence Obata Shuichi Department of Anatomy, Nagoya University, School of Medicine, 65 Tsurumai, Showa-ku, 466, Nagoya, Japan aut Usukura Jiro Department of Anatomy, Nagoya University, School of Medicine, 65 Tsurumai, Showa-ku, 466, Nagoya, Japan aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 39-48 0302-766X 269:1<39 1992 269 441 https://doi.org/10.1007/BF00384724 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384724 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Obata Shuichi Department of Anatomy, Nagoya University, School of Medicine, 65 Tsurumai, Showa-ku, 466, Nagoya, Japan aut NATIONALLICENCE 700 1- Usukura Jiro Department of Anatomy, Nagoya University, School of Medicine, 65 Tsurumai, Showa-ku, 466, Nagoya, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 39-48 0302-766X 269:1<39 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer