caa a22 4500 46908376X CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00384731 doi (NATIONALLICENCE)springer-10.1007/BF00384731 Morphological analysis of the neurons in the area of the hypothalamic magnocellular dorsal nucleus of the guinea pig [Elektronische Daten] [O. Doutrelant, L. Martin-Bouyer, P. Poulain] Summary: In the guinea-pig hypothalamus, a group of enkephalinergic cells forms a well-circumscribed nuclear area called the magnocellular dorsal nucleus (MDN). This nucleus gives rise to a prominent projection to the lateral spetum: the hypothalamo-septal enkephalinergic pathway. In the present study, MDN neurons visualized by Golgi impregnation were subjected to morphological analysis in order to define the potential segregation of cellular types within the MDN. This study was complemented by additional observations of MDN neurons intracellularly injected by Lucifer yellow (LY) or horseradish peroxidase (HRP) during the in vitro incubation of hypothalamic slices. The following results were obtained from the analysis of 200 neurons: 163 Golgi-impregnated cells plus 37 injected cells (LY=14; HRP=23). Thirteen HRP-injected cells were precisely located in the MDN and 10 were located in the perifornical area surrounding the MDN. Four different cellular types were identified. Type-I neurons (41%) displayed a globular perikaryon, a variable number of primary dendrites that were poorly ramified, no preferential orientation, and an axon emerging from the perikaryon. Type-II neurons (30.5%) had a triangular perikaryon, three well-ramified primary dendrites, an orientation perpendicular to the third ventricle, and an axon emerging from the perikaryon. Type-III neurons (22%) exhibited a spindle-shaped perikaryon, two opposed well-ramified primary dendrites, an orientation perpendicular to the third ventricle, and an axon emerging from a primary dendrite. Type-IV neurons (6.5%), showed a globular perikaryon, a variable number of primary dendrites, poorly ramified dendrites, an orientation parallel to the third ventricle, and an axon whose orientation could not be identified. Neurons labeled after intracellular injection belonged to the first three cellular types. Springer-Verlag, 1992 Hypothalamus nationallicence Magnocellular dorsal nucleus nationallicence Golgi impregnation nationallicence Horseradish, peroxidase nationallicence Lucifer-yellow labeling nationallicence Enkephalin nationallicence Guinea pig nationallicence Doutrelant O. INSERM U 156, Place de Verdun, F-59045, Lille Cedex, France aut Martin-Bouyer L. INSERM U 156, Place de Verdun, F-59045, Lille Cedex, France aut Poulain P. INSERM U 156, Place de Verdun, F-59045, Lille Cedex, France aut Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 107-117 0302-766X 269:1<107 1992 269 441 https://doi.org/10.1007/BF00384731 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00384731 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Doutrelant O. INSERM U 156, Place de Verdun, F-59045, Lille Cedex, France aut NATIONALLICENCE 700 1- Martin-Bouyer L. INSERM U 156, Place de Verdun, F-59045, Lille Cedex, France aut NATIONALLICENCE 700 1- Poulain P. INSERM U 156, Place de Verdun, F-59045, Lille Cedex, France aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/1(1992-07-01), 107-117 0302-766X 269:1<107 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer