caa a22 4500 469083786 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00353899 doi (NATIONALLICENCE)springer-10.1007/BF00353899 Ecto-ATPase/phosphatase activity in the olfactory sensilla of the spiny lobster, Panulirus argus [Elektronische Daten] localization and characterization [Richard Gleeson, Henry Trapido-Rosenthal, Lorraine McDowell, Henry Aldrich, William Carr] Summary: Electrophysiological studies have shown that the olfactory organ (antennule) of the spiny lobster, Panulirus argus, has chemoreceptors that are selectively excited by adenine nucleotides in seawater. Biochemical studies have revealed that these same nucleotides can be rapidly dephosphorylated by ectoenzymes associated with the olfactory sensilla (aesthetascs). In this study the distribution of ecto-ATPase/phosphatase activity within aesthetascs was determined cytochemically and the nature of the adenine-nucleotide dephosphorylating activity was dissected biochemically. Cytochemically, the distribution of ATP-dephosphorylating activity was similar to that shown previously for AMP and β-glycerol phosphate; i.e., cerium phosphate reaction product was specifically localized to the transitional zone where the sensory dendrites develop cilia and branch to form the outer dendritic segments. Unlike the dephosphorylation of AMP and β-glycerol phosphate, Mg2+ or Ca2+ was required for ecto-ATPase/phosphatase activity. Biochemical measures of both AMP-and ATP-dephosphorylating activity within aesthetascs corroborated the cytochemical evidence that these activities are localized to the transitional zone. A major portion of the AMP dephosphorylation (about 67%) derives from nonspecific alkaline phosphatase activity that is insensitive to levamisole and L-bromotetramisole. In contrast, nonspecific phosphatase activity accounted for a much smaller part of the ATP dephosphorylation (about 15%). Ectoenzymatic activity in the transitional zone may be an important means of removing excitatory/inhibitory nucleotides from this region. Springer-Verlag, 1992 Aesthetasc nationallicence Adenosine triphosphate nationallicence Cerium phosphate nationallicence Cytochemistry nationallicence Ectonucleotidase (Crustacea) nationallicence Olfactory system nationallicence Phosphatase nationallicence Panulirus argus nationallicence ADP : Adenosine 5′-diphosphate nationallicence AMP : adenosine 5′-monophosphate nationallicence AMPCP : α, β-methylene ADP nationallicence ASW : artificial seawater nationallicence ATP : adenosine 5′-triphosphate nationallicence β-GP : β-glycerol phosphate nationallicence EM : electron microscopy nationallicence Gleeson Richard The Whitney Laboratory and Department of Zoology, University of Florida, 9505 Ocean Shore Boulevard, 32086, St. Augustine, FL, USA aut Trapido-Rosenthal Henry The Whitney Laboratory and Department of Zoology, University of Florida, 9505 Ocean Shore Boulevard, 32086, St. Augustine, FL, USA aut McDowell Lorraine Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA aut Aldrich Henry Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA aut Carr William The Whitney Laboratory and Department of Zoology, University of Florida, 9505 Ocean Shore Boulevard, 32086, St. Augustine, FL, USA aut Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 439-445 0302-766X 269:3<439 1992 269 441 https://doi.org/10.1007/BF00353899 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353899 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gleeson Richard The Whitney Laboratory and Department of Zoology, University of Florida, 9505 Ocean Shore Boulevard, 32086, St. Augustine, FL, USA aut NATIONALLICENCE 700 1- Trapido-Rosenthal Henry The Whitney Laboratory and Department of Zoology, University of Florida, 9505 Ocean Shore Boulevard, 32086, St. Augustine, FL, USA aut NATIONALLICENCE 700 1- McDowell Lorraine Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA aut NATIONALLICENCE 700 1- Aldrich Henry Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA aut NATIONALLICENCE 700 1- Carr William The Whitney Laboratory and Department of Zoology, University of Florida, 9505 Ocean Shore Boulevard, 32086, St. Augustine, FL, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 439-445 0302-766X 269:3<439 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer