caa a22 4500 469083794 CHVBK 20180323132958.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00353895 doi (NATIONALLICENCE)springer-10.1007/BF00353895 Correlation of extracellular matrix components with the cytoarchitecture of mouse Peyer's patches [Elektronische Daten] [Aiji Ohtsuka, Anthony Piazza, Thomas Ermak, Robert Owen] Summary: The distribution patterns of extracellular matrix elements were determined to ascertain whether they play a role in the localization of lymphocytes in discrete T-cell, B-cell and dome antigen-processing domains within Peyer's patches. Antibodies against collagen types I, III and IV, laminin and fibronectin were applied to cryosections of mouse Peyer's patches and localized by direct or indirect immunoperoxidase methods. T-cell domains were identified with a monoclonal antibody against Thy-1.2. Labeled reticular fibers in distinctive patterns were more numerous in parafollicular and dome areas than within follicles. Germinal centers contained few such fibers. In parafollicular areas, fibers were oriented predominantly toward follicle domes; their distribution corresponded to T-cell zones and lymphocyte traffic areas, with their orientation being parallel to the migration pathways of lymphocytes from high endothelial venules to the antigen-processing domes. Subepithelial and subendothelial basal laminae were immunopositive for type-IV collagen, laminin and fibronectin. The dome subepithelial basal lamina had pore-like discontinuities through which lymphocytes migrated to and from the epithelium. The correspondence of the distribution patterns of extracellular matrix to specific functional domains of Peyer's patches suggests that this matrix provides a structural framework for lymphocyte migration and localization. Springer-Verlag, 1992 Peyer's patches nationallicence Collagen nationallicence Laminin nationallicence Fibronectin nationallicence Extracellular matrix nationallicence Lymphocytes nationallicence Immunohistochemistry nationallicence Mouse (BALB/c) nationallicence Ohtsuka Aiji Cell Biology and Aging Section, VA Medical Center, and Department of Medicine, School of Medicine, University of California, 94121, San Francisco, CA, USA aut Piazza Anthony Cell Biology and Aging Section, VA Medical Center, and Department of Medicine, School of Medicine, University of California, 94121, San Francisco, CA, USA aut Ermak Thomas Cell Biology and Aging Section, VA Medical Center, and Department of Medicine, School of Medicine, University of California, 94121, San Francisco, CA, USA aut Owen Robert Cell Biology and Aging Section, VA Medical Center, and Department of Medicine, School of Medicine, University of California, 94121, San Francisco, CA, USA aut Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 403-410 0302-766X 269:3<403 1992 269 441 https://doi.org/10.1007/BF00353895 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353895 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohtsuka Aiji Cell Biology and Aging Section, VA Medical Center, and Department of Medicine, School of Medicine, University of California, 94121, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Piazza Anthony Cell Biology and Aging Section, VA Medical Center, and Department of Medicine, School of Medicine, University of California, 94121, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Ermak Thomas Cell Biology and Aging Section, VA Medical Center, and Department of Medicine, School of Medicine, University of California, 94121, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Owen Robert Cell Biology and Aging Section, VA Medical Center, and Department of Medicine, School of Medicine, University of California, 94121, San Francisco, CA, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 403-410 0302-766X 269:3<403 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer