caa a22 4500 469083816 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00353906 doi (NATIONALLICENCE)springer-10.1007/BF00353906 Immunocytochemical and circadian biochemical analysis of neuroactive amino acids in the pineal gland of the rat: effect of superior cervical ganglionectomy [Elektronische Daten] [J. McNulty, L. Kus, O. Ottersen] Summary: Semiquantitative immunocytochemistry by immuno-gold techniques revealed differences in the spatial distribution of glutamate, glutamine, and taurine within the pineal gland, with greatest labeling over pinealocytes, glia, and endothelia, respectively. At the subcellular level, glutamate labeling tended to be highest over pinealocyte synaptic ribbons and mitochondria, and lowest over lipid inclusions. Pineal levels of glutamate, glutamine and taurine, as measured by high performance liquid chromatography, did not vary over a light: dark cycle. Superior cervical sympathetic denervation, which abolishes pineal melatonin synthesis, resulted in a nearly 50% reduction in pineal glutamate levels, but had no effect on levels of glutamine and taurine. Other amino acids (alanine, arginine, aspartate, serine) were reduced by 23%-33% following sympathectomy. These data suggest an important role for glutamate in pinealocyte function(s) possibly related to the noradrenergic innervation of the gland. Springer-Verlag, 1992 Pineal gland nationallicence Pinealocytes nationallicence Glutamate nationallicence Glutamine nationallicence Taurine nationallicence Circadian rhythm nationallicence Superior cervical sympathectomy nationallicence Rat (Sprague-Dawley) nationallicence McNulty J. Department of Cell Biology, Neurobiology and Anatomy, Loyola University, Stritch School of Medicine, 2160 S. First Avenue, 60153, Maywood, IL, USA aut Kus L. Department of Cell Biology, Neurobiology and Anatomy, Loyola University, Stritch School of Medicine, 2160 S. First Avenue, 60153, Maywood, IL, USA aut Ottersen O. Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Sognsvannsv. 9, P.O. Box 1105, N-0317, Oslo 3, Norway aut Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 515-523 0302-766X 269:3<515 1992 269 441 https://doi.org/10.1007/BF00353906 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353906 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McNulty J. Department of Cell Biology, Neurobiology and Anatomy, Loyola University, Stritch School of Medicine, 2160 S. First Avenue, 60153, Maywood, IL, USA aut NATIONALLICENCE 700 1- Kus L. Department of Cell Biology, Neurobiology and Anatomy, Loyola University, Stritch School of Medicine, 2160 S. First Avenue, 60153, Maywood, IL, USA aut NATIONALLICENCE 700 1- Ottersen O. Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Sognsvannsv. 9, P.O. Box 1105, N-0317, Oslo 3, Norway aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 515-523 0302-766X 269:3<515 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer