caa a22 4500 469083832 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00353900 doi (NATIONALLICENCE)springer-10.1007/BF00353900 Species differences in the immunoreactive patterns of calcitonin gene-related peptide in the pancreas [Elektronische Daten] [C. Sternini, R. De Giorgio, K. Anderson, P. Watt, F. Brunicardi, A. Widdison, H. Wong, H. Reber, J. Walsh, V. Go] Summary: In the pancreas, calcitonin gene-related peptide (CGRP) immunoreactivity has been described in nerve fibers and in distinct types of islet cells. This unique, apparently species-specific cell-type expression prompted the present investigation to clarify further the pattern of CGRP immunoreactivity in different mammalian species (i.e., different strains of rats, mice, guinea pigs, rabbits, cats, dogs, pigs, and humans) commonly used for functional and anatomical studies of the pancreas by means of immunohistochemistry using three different CGRP antibodies. In each species, CGRP-immunoreactive neurites innervate the exocrine and endocrine compartments, the vasculature, and the intrapancreatic ganglia, where they form dense networks encircling unstained cell bodies. The only exception is the pig pancreas, where the islets appear to be devoid of immunoreactive fibers. The overall density of immunoreactive pancreatic axons in different species is as follows: rat, mouse, and rabbit>guinea pig≥pig and cat> >dog and human. CGRP-immunoreactive endocrine cells appear to be restricted to the rat pancreas, where they form a subpopulation of somatostatin-containing D cells. In contrast, in mouse, guinea pig, cat, dog, and human pancreas, a homogeneous staining of the core of the islets, where insulin-producing B cells are located, was visualized in sections incubated with the rabbit CGRP antiserum at 4°C, but not at 37°C (an incubation temperature that does not affect the islet cell staining in the rat nor the fiber labeling in any species). Furthermore, the staining of islet B cells was not reproductible with all the CGRP antibodies used, all of which comparably stain nerve fibers in each species, and islet D cells in the rat. Immunoreactive islet cells were not visualized in pig and rabbit pancreas. These results are consistent with the hypothesis that the expression of CGRP in nerve fibers is a common feature of mammalian pancreas, whereas its expression in endocrine cells appears to be restricted to the D cells of the rat pancreas. Springer-Verlag, 1992 Amylin nationallicence Calcitonin gene-related peptide nationallicence Exocrine and endocrine pancreas nationallicence Insulin nationallicence Islet cells nationallicence Nerve fibers nationallicence Somatostatin nationallicence Mammals nationallicence Sternini C. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut De Giorgio R. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut Anderson K. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut Watt P. Department of Surgery, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut Brunicardi F. Department of Surgery, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut Widdison A. Department of Surgery, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut Wong H. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut Reber H. Department of Surgery, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut Walsh J. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut Go V. Department of Medicine, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 447-458 0302-766X 269:3<447 1992 269 441 https://doi.org/10.1007/BF00353900 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353900 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sternini C. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- De Giorgio R. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Anderson K. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Watt P. Department of Surgery, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Brunicardi F. Department of Surgery, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Widdison A. Department of Surgery, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Wong H. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Reber H. Department of Surgery, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Walsh J. Center for Ulcer Research and Education/Digestive Disease Center, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Go V. Department of Medicine, UCLA, School of Medicine, 90073, Los Angeles, CA, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 447-458 0302-766X 269:3<447 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer