caa a22 4500 469083840 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00353908 doi (NATIONALLICENCE)springer-10.1007/BF00353908 Functional and morphological changes associated with the ageing of primary cultures of embryonic adrenal gland cells derived from the Pekin duck [Elektronische Daten] [James Cronshaw, M. Collie, W. Holmes] Summary: The morphological and functional changes associated with ageing were studied in adrenal steroidogenic cells derived from duck embryos. Cells grown for not more than three days had structural characteristics similar to their counterparts in vivo; they contained numerous lipid droplets and mitochondria, an abundant smooth edoplasmic reticulum, an even network of microtubules, and microfilaments that formed extensive and elaborate systems of parallel stress fibers. After the 3rd day of growth in culture, many of the cells started to decrease in size and become elongated; the older cells showed less well-defined actin filaments and contained elongated mitochondria, fewer lipid droplets, less smooth endoplasmic reticulum, and swollen cisternae of rough endoplasmic reticulum. The proliferative capacity of the cells was the same when they were cultured in either the presence or the absence of 1-24 ACTH. After the first day of growth in culture, the steroidogenic capacity of the cells declined and the addition of 1-24 ACTH to the growth medium did not prevent changes in their structure and function. The decline in steroidogenic capacity occurred both in terms of the amount of hormone released into the culture medium and in the ability of the cells to respond when incubated in buffer containing 1-24 ACTH. Since the basal unstimulated rates of corticosteroid production also declined as the cells aged, it is probable that the steroidogenic deficiency occurs at a site distal to the corticotropin receptor; this is also consistent with the ultrastructural observations that suggest a relationship between the morphological changes and the decline in steroidogenic capacity as the cells age. Springer-Verlag, 1992 Adrenal gland nationallicence Cell culture nationallicence ACTH nationallicence Ageing nationallicence Corticosteroid nationallicence Cytoskeleton nationallicence Domestic mallard, embryos nationallicence Cronshaw James Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut Collie M. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut Holmes W. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 535-545 0302-766X 269:3<535 1992 269 441 https://doi.org/10.1007/BF00353908 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353908 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cronshaw James Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut NATIONALLICENCE 700 1- Collie M. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut NATIONALLICENCE 700 1- Holmes W. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 535-545 0302-766X 269:3<535 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer