caa a22 4500 469083859 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00353909 doi (NATIONALLICENCE)springer-10.1007/BF00353909 Tunicamycin, puromycin and brefeldin A influence the subcellular distribution of neuropeptides in hypothalamic magnocellular neurones of rat [Elektronische Daten] [David Pow, John Morris] Summary: Magnocellular neurones in the supraoptic nuclei of normal Long Evans and homozygous Brattleboro rats were examined electron-microscopically after intracisternal injections of tunicamycin, puromycin, or brefeldin A. Moderate (50 μg) or high (200 μg) doses of tunicamycin caused the formation of electron-dense filamentous accretions in the endoplasmic reticulum (ER) cisterns of vasopressin neurones, but only the high dose of tunicamycin also caused accretions to form in the ER of some oxytocin neurones. Immunogold labelling of ultrathin sections from tunicamycin-treated rats revealed that, in about 5% of vasopressin neurones, the accretions could be immunogold-labelled for vasopressin and its associated neurophysin. However, in the majority of vasopressin neurones, the sections required trypsinisation before immunolabelling of the accretions could be detected. Small accretions in the ER of oxytocin neurones did not label for oxytocin or its neurophysin without prior trypsinisation, whereas larger accretions in other oxytocin cells could be labelled without prior trypsin treatment. Administration of puromycin resulted in the formation of small ER accretions in both vasopressin and oxytocin neurones. These accretions were immunolabelled with antisera, respectively, to vasopressin and oxytocin, but neurophysin-immunoreactivity was in most cases absent and was not revealed by treatment with trypsin, suggesting that neurophysin-immunoreactive epitopes were absent from truncated peptides forming the accretions. Brefeldin A caused dilatation of ER cisterns and disruption of the Golgi apparatus in both oxytocin and vasopressin neurones, but did not cause accretions to form in the ER. Springer-Verlag, 1992 Neurosecretion nationallicence Tunicamycin nationallicence Puromycin nationallicence Brefeldin A nationallicence Vasopressin nationallicence Oxytocin nationallicence Neurophysin nationallicence Rat (Long Evans, Brattleboro) nationallicence Pow David Department of Human Anatomy, University of Oxford, South Parks Road, OX1 3QX, Oxford, UK aut Morris John Department of Human Anatomy, University of Oxford, South Parks Road, OX1 3QX, Oxford, UK aut Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 547-560 0302-766X 269:3<547 1992 269 441 https://doi.org/10.1007/BF00353909 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353909 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pow David Department of Human Anatomy, University of Oxford, South Parks Road, OX1 3QX, Oxford, UK aut NATIONALLICENCE 700 1- Morris John Department of Human Anatomy, University of Oxford, South Parks Road, OX1 3QX, Oxford, UK aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 547-560 0302-766X 269:3<547 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer