caa a22 4500 469083883 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00353896 doi (NATIONALLICENCE)springer-10.1007/BF00353896 Electron microscopy of a double helical tubular filament in keyhole limpet ( Megathura crenulata ) hemolymph [Elektronische Daten] [J. Harris, Jürgen Markl] Summary: A ≈25 nm hollow double helical filament has been detected ultrastructurally in the cell-free supernatant from hemolymph of the keyhole limpet Megathura crenulata (Gastropoda: Prosobranchia: Fissurellidae). Subsequently, much higher concentrations of this material were found in the cell pellet from hemolymph. Both negative staining and thin sectioning have been performed in an attempt to obtain a preliminary structural characterization of this new filament. It is proposed that the filaments are released or secreted from blood hemocytes in response to bleeding, but it has not been possible to define absolutely an intracellular organelle containing this material. It is shown that the hollow filaments, which have fine fibrillar surface extensions, are readily distinguishable from collagen fibrils and from tubular (multi-decameric) keyhole limpet hemocyanin (KLH). Springer-Verlag, 1992 Hemolymph nationallicence Filaments nationallicence Hemocyanin nationallicence Hemocytes nationallicence Megathura crenulata (Mollusca) nationallicence Harris J. Institut für Zell- und Tumorbiologic, Deutsches Krebsforschungszentrum, W-6900, Heidelberg, Federal Republic of Germany aut Markl Jürgen Institut für Zoologie der Universität, W-6500, Mainz, Federal Republic of Germany aut Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 411-420 0302-766X 269:3<411 1992 269 441 https://doi.org/10.1007/BF00353896 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353896 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harris J. Institut für Zell- und Tumorbiologic, Deutsches Krebsforschungszentrum, W-6900, Heidelberg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Markl Jürgen Institut für Zoologie der Universität, W-6500, Mainz, Federal Republic of Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 411-420 0302-766X 269:3<411 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer