caa a22 4500 469083891 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00353898 doi (NATIONALLICENCE)springer-10.1007/BF00353898 Martin Vicki Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, IN, USA aut Characterization of a RFamide-positive subset of ganglionic cells in the hydrozoan planular nerve net [Elektronische Daten] [Vicki Martin] Summary: The complexity of the hydrozoan planular nervous system was examined. Using a whole-mount technique with indirect immunofluorescence, the spatial pattern of ganglionic cells showing RFamide-like immunoreactivity was visualized. RFamide antiserum bound a subset of ganglionic cells in the anterior and upper middle regions of the planula and a few ganglionic cells in the upper tail region. Labeled cells consisted of bipolar and multipolar neurons. Stained processes from these cells formed a three-dimensional nerve net that followed the contour of the mesoglea; such fibers were striking in terms of their large numbers, long lengths, and organization into distinct bundles. Labeled fibers were seen to contact other ganglionic cells, sensory cells, epitheliomuscle cells, the mesoglea, and the outside free surface. All stained cell bodies and fibers were found in the ectoderm. Using the same technique the reappearance of RFamide-positive ganglionic cells in epithelial tissue of chimeric grafts of planulae was observed. Interstitial cells capable of forming RFamide-positive ganglionic cells underwent extensive anterior-posterior migrations in the grafts, moved into the epithelial tissue, and differentiated into RFamide-positive ganglionic cells. Stained repopulated ganglionic cells always formed in the same position in the epithelial tissue as was observed in control planulae suggesting that the expression of RFamide-like substances may be position dependent in the planula. Springer-Verlag, 1992 Nervous system (Hydrozoa, Planula) nationallicence RFamide nationallicence Nerve net nationallicence Neuropeptide nationallicence Pennaria tiarella (Cnidaria) nationallicence Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 431-438 0302-766X 269:3<431 1992 269 441 https://doi.org/10.1007/BF00353898 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353898 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Martin Vicki Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, IN, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/3(1992-09-01), 431-438 0302-766X 269:3<431 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer