caa a22 4500 469083964 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319613 doi (NATIONALLICENCE)springer-10.1007/BF00319613 Splenic primary sensory afferents in the guinea pig demonstrated with anterogradely transported wheat-germ agglutinin conjugated to horseradish peroxidase [Elektronische Daten] [Lars-Gösta Elfvin, Håkan Aldskogius, Jan Johansson] Summary: The distribution of primary visceral afferents to the spleen of the guinea pig was studied after injections of wheat-germ agglutinin conjugated to horseradish peroxidase (HRP) into the left dorsal root ganglia at levels T7-T12. After anterograde transport of the tracer, labeled fibers were found in the nerves around the splenic artery in the hilus region and in the splenic parenchyma. The majority of labeled fibers in the spleen were detected in the white pulp. HRP-positive fibers were also observed in the red pulp and in the trabeculae. The distribution of the HRP-labeled fibers was in part similar to those of substance P-immunoreactive and calcitonin gene-related peptide-immunoreactive nerve structures. The results show that the anterograde tracing technique can be used successfully to investigate splenic primary afferent innervation. Springer-Verlag, 1992 Spleen nationallicence Splenic nerve nationallicence Primary sensory neurons nationallicence Lectin nationallicence Horseradish peroxidase conjugate nationallicence Anterograde axonal transport nationallicence Guinea pig nationallicence Elfvin Lars-Gösta Department of Anatomy, Karolinska Institutet, P.O. Box 60400, S-104 01, Stockholm, Sweden aut Aldskogius Håkan Department of Anatomy, Karolinska Institutet, P.O. Box 60400, S-104 01, Stockholm, Sweden aut Johansson Jan Department of Anatomy, Karolinska Institutet, P.O. Box 60400, S-104 01, Stockholm, Sweden aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 229-234 0302-766X 269:2<229 1992 269 441 https://doi.org/10.1007/BF00319613 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319613 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Elfvin Lars-Gösta Department of Anatomy, Karolinska Institutet, P.O. Box 60400, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 700 1- Aldskogius Håkan Department of Anatomy, Karolinska Institutet, P.O. Box 60400, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 700 1- Johansson Jan Department of Anatomy, Karolinska Institutet, P.O. Box 60400, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 229-234 0302-766X 269:2<229 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer