caa a22 4500 469083980 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319618 doi (NATIONALLICENCE)springer-10.1007/BF00319618 Pituitary adenylate cyclase-activating polypeptide (PACAP): occurrence in rodent pancreas and effects on insulin and glucagon secretion in the mouse [Elektronische Daten] [Tord Fridolf, Frank Sundler, Bo Ahrén] Summary: Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide that occurs in several tissues, e.g., in the gut. We have studied PACAP-like immunoreactivity in the pancreas of rat and mouse, and the effects of PACAP-38 on basal and stimulated insulin and glucagon secretion in the mouse. Immunofluorescence staining demonstrated the presence of PACAP-like immunoreactivity in nerve fibers in both the rat and mouse pancreas. The nerve fibers were seen in the exocrine pancreas and surrounding the islets. Occasionally, the nerve fibers occurred within the islets. Most PACAP-positive nerve fibers innervated the intrapancreatic ganglia, although no nerve cell bodies contained PACAP-like immunoreactivity. In-vivo experiments in mice revealed that basal plasma glucagon levels were increased by PACAP-39 injected intravenously at dose levels exceeding 1.8 nmol/kg. Furthermore, PACAP-38 (7 nmol/kg) potentiated the plasma glucagon response to the cholinergic agonist carbachol (0.16 μmol/kg). This potentiation was reduced to simple addition by pretreatment with a combined α- and β-adrenergic blockade by phentolamine (35 μmol/kg) and propranolol (8.5 μmol/kg). Moreover, PACAP-38 inhibited a carbachol-induced increase in the level of plasma insulin in the absence but not in the presence of adrenergic blockade. PACAP-38 increased basal plasma insulin levels and increased basal plasma glucose levels 6 min and 10 min, respectively, after injection of the peptide. We conclude that PACAP-like immunoreactivity exists in nerve fibers innervating the mouse and rat pancreas, particularly the intrapancreatic ganglia, and that PACAP-38 augments both basal and carbachol-stimulated glucagon secretion in the mouse. Springer-Verlag, 1992 Pituitary adenylate cyclase-activating peptide (PACAP) nationallicence Immunocytochemistry nationallicence Pancreas, endocrine, exocrine nationallicence Insulin secretion nationallicence Glucagon secretion nationallicence Mouse (NMRI) nationallicence Rat (Sprague-Dawley) nationallicence Fridolf Tord Department of Pharmacology, Lund University, Sölyegatan 10, S-223 62, Lund, Sweden aut Sundler Frank Department of Medical Cell Research, Lund University, Biskopsgatan 5, S-223 62, Lund, Sweden aut Ahrén Bo Department of Surgery, Lund University, S-223 62, Lund, Sweden aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 275-279 0302-766X 269:2<275 1992 269 441 https://doi.org/10.1007/BF00319618 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319618 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fridolf Tord Department of Pharmacology, Lund University, Sölyegatan 10, S-223 62, Lund, Sweden aut NATIONALLICENCE 700 1- Sundler Frank Department of Medical Cell Research, Lund University, Biskopsgatan 5, S-223 62, Lund, Sweden aut NATIONALLICENCE 700 1- Ahrén Bo Department of Surgery, Lund University, S-223 62, Lund, Sweden aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 275-279 0302-766X 269:2<275 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer