caa a22 4500 469084049 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319630 doi (NATIONALLICENCE)springer-10.1007/BF00319630 A novel neuropeptide, pituitary adenylate cyclase-activating polypeptide (PACAP), in human intestine: evidence for reduced content in Hirschsprung's disease [Elektronische Daten] [Zhenxin Shen, Lars Larsson, Gerhard Malmfors, Afaf Absood, Rolf Håkanson, Frank Sundler] Summary: A novel neuropeptide, pituitary adenylate cyclase-activating polypeptide (PACAP), exhibits sequence homology with vasoactive intestinal polypeptide (VIP) and occurs in the mammalian brain, lung and gut. The distribution of PACAP in ganglionic and aganglionic portions of the large intestine of patients with Hirschsprung's disease was examined by immunohistochemistry and radioimmunoassay. PACAP-immunoreactive nerve fibers were distributed in all layers of the ganglionic and aganglionic segments of the intestine, although they were less numerous in the latter, and PACAP-immunoreactive nerve cell bodies were seen in the ganglionic portion of the intestine. The concentration of immunoreactive PACAP was lower in the aganglionic than in the ganglionic segment of the intestinal wall. PACAP and VIP were found to coexist in both ganglionic and aganglionic segments of the intestine. Apparently, PACAP participates in the regulation of gut motility. The scarcer PACAP innervation of the aganglionic segment may contribute to the defect in intestinal relaxation seen in patients with Hirschsprung's disease. Springer-Verlag, 1992 Pituitary adenylate cyclase-activating polypeptide (PACAP) nationallicence Vasoactive intestinal polypeptide nationallicence Neuropeptide immunocytochemistry nationallicence Radioimmunoassay nationallicence Hirschsprung's disease nationallicence Intestine, large nationallicence GUT motility nationallicence Human nationallicence Shen Zhenxin Department of Pediatric Surgery, University of Lund, S-22185, Lund, Sweden aut Larsson Lars Department of Pediatric Surgery, University of Lund, S-22185, Lund, Sweden aut Malmfors Gerhard Department of Pediatric Surgery, University of Lund, S-22185, Lund, Sweden aut Absood Afaf Department of Pharmacology, University of Lund, S-22185, Lund, Sweden aut Håkanson Rolf Department of Pharmacology, University of Lund, S-22185, Lund, Sweden aut Sundler Frank Department of Medical Cell Research, University of Lund, S-22185, Lund, Sweden aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 369-374 0302-766X 269:2<369 1992 269 441 https://doi.org/10.1007/BF00319630 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319630 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Zhenxin Department of Pediatric Surgery, University of Lund, S-22185, Lund, Sweden aut NATIONALLICENCE 700 1- Larsson Lars Department of Pediatric Surgery, University of Lund, S-22185, Lund, Sweden aut NATIONALLICENCE 700 1- Malmfors Gerhard Department of Pediatric Surgery, University of Lund, S-22185, Lund, Sweden aut NATIONALLICENCE 700 1- Absood Afaf Department of Pharmacology, University of Lund, S-22185, Lund, Sweden aut NATIONALLICENCE 700 1- Håkanson Rolf Department of Pharmacology, University of Lund, S-22185, Lund, Sweden aut NATIONALLICENCE 700 1- Sundler Frank Department of Medical Cell Research, University of Lund, S-22185, Lund, Sweden aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 369-374 0302-766X 269:2<369 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer