caa a22 4500 469084057 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319625 doi (NATIONALLICENCE)springer-10.1007/BF00319625 Co-localization of vimentin and cytokeratins in M-cells of rabbit gut-associated lymphoid tissue (GALT) [Elektronische Daten] [Andreas Gebert, Gudrun Hach, Helmut Bartels] Summary: The occurrence of cytokeratins, vimentin, and desmin in the dome epithelia and adjacent non-dome epithelia in four locations of gut-associated lymphoid tissues (GALT) of adult and newborn rabbits (Peyer's patches, sacculus rotundus, caecal lymphoid patches and appendix) was studied with monoclonal antibodies, using the indirect immunoperoxidase technique. In all locations investigated in adult animals, antibodies specific for vimentin labelled (1) M-cells, which engulf intraepithelial lymphocytes, (2) columnar epithelial cells at the base of the domes lacking an apparent contact with lymphocytes ("immature” M-cells), and (3) flat cells, which lie in the lamina propria under the dome epithelium, and which line the basal lamina with thin cytoplasmic processes. In newborn rabbits, columnar epithelial cells resembling the immature M-cells of adults were selectively stained with vimentin antibodies. In M-cells, the strongest immunoreactivity was present in the perinuclear region and close to the pocket membrane, whereas the most apical and most basal parts of the cytoplasm showed no vimentin-immunoreactivity. Enterocytes in the dome epithelium and in the non-dome epithelium were vimentin-negative. M-cells and enterocytes bound antibodies against cytokeratin peptides 18 and 19 in adults and newborn animals. Compared with enterocytes, M-cells showed less intense staining for cytokeratins. Dome epithelia and no-dome epithelia did not contain desmin-immunoreactive cells. The results suggest that vimentin is a sensitive marker for M-cells in rabbit GALT. Springer-Verlag, 1992 M-cells nationallicence Vimentin nationallicence Cytokeratins nationallicence Peyer's patches nationallicence Appendix nationallicence Gut-associated lymphoid tissue (GALT) nationallicence Rabbit nationallicence Gebert Andreas Lehrstuhl II, Anatomische Anstalt der Universität München, Pettenkoferstrasse 11, W-8000, München 2, Federal Republic of Germany aut Hach Gudrun Lehrstuhl II, Anatomische Anstalt der Universität München, Pettenkoferstrasse 11, W-8000, München 2, Federal Republic of Germany aut Bartels Helmut Abteilung Zellbiologie und Elektronenmikroskopie, Medizinische Hochschule Hannover, W-3000, Hannover 61, Federal Republic of Germany aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 331-340 0302-766X 269:2<331 1992 269 441 https://doi.org/10.1007/BF00319625 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319625 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gebert Andreas Lehrstuhl II, Anatomische Anstalt der Universität München, Pettenkoferstrasse 11, W-8000, München 2, Federal Republic of Germany aut NATIONALLICENCE 700 1- Hach Gudrun Lehrstuhl II, Anatomische Anstalt der Universität München, Pettenkoferstrasse 11, W-8000, München 2, Federal Republic of Germany aut NATIONALLICENCE 700 1- Bartels Helmut Abteilung Zellbiologie und Elektronenmikroskopie, Medizinische Hochschule Hannover, W-3000, Hannover 61, Federal Republic of Germany aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 331-340 0302-766X 269:2<331 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer