caa a22 4500 469084065 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319623 doi (NATIONALLICENCE)springer-10.1007/BF00319623 Localization of calcitonin gene-related peptide and islet amyloid polypeptide in the rat and mouse pancreas [Elektronische Daten] [Bo Ahrén, Frank Sundler] Summary: It was previously demonstrated that the two chemically related peptides calcitonin gene-related peptide (CGRP) and islet amyloid polypeptide (IAPP) both occur in the pancreas. We have now examined the cellular localization of CGRP and IAPP in the rat and the mouse pancreas. We found, in both the rat and the mouse pancreas, CGRP-immunoreactive nerve fibers throughout the parenchyma, including the islets, with particular association with blood vessels. CGRP-immunoreactive nerve fibers were regularly seen within the islets. In contrast, no IAPP-immunoreactive nerve fibers were demonstrated in this location. Furthermore, in rat islets, CGRP immunoreactivity was demonstrated in peripherally located cells, constituting a major subpopulation of the somatostatin cells. Such cells were lacking in the mouse islets. IAPP-like immunoreactivity was demonstrated in rat and mouse islet insulin cells, and, in the rat, also in a few non-insulin cells in the islet periphery. These cells seemed to be identical with somatostatin/CGRP-immunoreactive elements. In summary, the study shows (1) that CGRP, but not IAPP, is a pancreati neuropeptide both in the mouse and the rat; (2) that a subpopulation of rat somatostatin cells contain CGRP; (3) that mouse islet endocrine cells do not contain CGRP; (4) that insulin cells in both the rat and the mouse contain IAPP; and (5) that in the rat, a non-insulin cell population apparently composed of somatostatin cells stores immunoreactive IAPP. We conclude that CGRP is a pancreatic neuropeptide and IAPP is an islet endocrine peptide in both the rat and the mouse, whereas CGRP is an islet endocrine peptide in the rat. Springer-Verlag, 1992 Calcitonin gene-related peptide nationallicence Islet amyloid polypeptide nationallicence Immunocytochemistry nationallicence Pancreas endocrine, exocrine nationallicence Rat (Sprague-Dawley) nationallicence Mouse (NMRI) nationallicence Ahrén Bo Department of Surgery, Lund University, S-221 85, Lund, Sweden aut Sundler Frank Department of Medical Cell Research, Lund University, S-221 85, Lund, Sweden aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 315-322 0302-766X 269:2<315 1992 269 441 https://doi.org/10.1007/BF00319623 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319623 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahrén Bo Department of Surgery, Lund University, S-221 85, Lund, Sweden aut NATIONALLICENCE 700 1- Sundler Frank Department of Medical Cell Research, Lund University, S-221 85, Lund, Sweden aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 315-322 0302-766X 269:2<315 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer