caa a22 4500 469084081 CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319614 doi (NATIONALLICENCE)springer-10.1007/BF00319614 In vivo effects of tunicamycin on chondrocytes of rat mandibular condyles as revealed by lectin cytochemistry [Elektronische Daten] [Satoshi Yokose, Yoshifumi Tajima] Summary: The in vivo effects of tunicamycin on the glycosylation of proteoglycans and link protein in rat mandibular condylar chondrocytes were studied by ultrastructural lectin histochemistry. The binding of wheatgerm agglutinin was shown by using anti-lectin antibody followed by protein A-gold complex. In normal rats, wheat-germ agglutinin labeling was restricted to trans cisternae and vacuoles of the Golgi complex, whereas it was observed in neither the cis region of the Golgi complex nor in the rough endoplasmic reticulum. By 3 h after the drug administration, wheat-germ agglutinin binding sites on the disorganized Golgi vacuoles were dramatically reduced in number. At 6 h after the drug administration, the lectin binding sites on the Golgi vacuoles were restored. These results demonstrate that the in vivo use of tunicamycin in combination with histochemical analysis using lectin probes is of significant value for the study of protein glycosylation in chondrocytes of the rat mandibular condyle. Springer-Verlag, 1992 Tunicamycin nationallicence Chondrocytes nationallicence Mandibular condyle nationallicence Wheat-germ agglutinin nationallicence Rat (Sprague-Dawley) nationallicence Yokose Satoshi Department of Oral Pathology, Meikai University School of Dentistry, 350-02, Sakado, Saitama, Japan aut Tajima Yoshifumi Department of Oral Pathology, Meikai University School of Dentistry, 350-02, Sakado, Saitama, Japan aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 235-239 0302-766X 269:2<235 1992 269 441 https://doi.org/10.1007/BF00319614 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319614 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yokose Satoshi Department of Oral Pathology, Meikai University School of Dentistry, 350-02, Sakado, Saitama, Japan aut NATIONALLICENCE 700 1- Tajima Yoshifumi Department of Oral Pathology, Meikai University School of Dentistry, 350-02, Sakado, Saitama, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 235-239 0302-766X 269:2<235 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer