caa a22 4500 46908409X CHVBK 20180323132959.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319608 doi (NATIONALLICENCE)springer-10.1007/BF00319608 Postnatal development and testosterone-dependence of GP-83 and GP-49, two sperm maturation-related glycoproteins in BALB/c mouse epididymis [Elektronische Daten] [Hwan-Wun Liu, Guang-Huan Sun, Shyi-Ren Shy, Hann-Yeh Shyu] Summary: The secretion of sperm maturation-related molecules by the epididymis is subjected to developmental and hormonal regulation. In the BALB/c mouse, we found that GP-83 and GP-49, two sperm maturationrelated glycoproteins, were secreted by the epididymis. The present study investigated the postnatal development and testosterone-dependence of these two molecules. Histochemical localization in paraffin sections revealed that wheat-germ agglutinin (WGA)-binding sites were first present in the epididymis of 4-week-old mice. The distribution of WGA-binding sites was the same as that of more mature mice, i.e., it was first found in the principal cells of the corpus epididymidis, and gradually appeared in the contents of epididymal tubules. On WGA blots, GP-83 and GP-49 were identified in the corpus, and GP-83 was identified in the cauda of the epididymis. In mice that had received unilateral orchiectomy at 4 weeks of age, GP-83 and GP-49 were present in both intact and orchiectomized epididymides 4 weeks after the operation. In the epididymides of mice that had received bilateral orchiectomy, GP-83 and GP-49 were barely identifiable. However, the presence of these two molecules was restored if testosterone was supplemented immediately after orchiectomy. These results indicate that GP-83 and GP-49 are secreted de novo in the epididymis, and that the secretion of these two molecules is developmentally regulated and androgen-dependent. Springer-Verlag, 1992 Epididymis nationallicence Sperm maturation nationallicence Androgen dependence nationallicence Developmental regulation nationallicence Sperm antigens nationallicence Mouse (BALB/c) nationallicence Liu Hwan-Wun Department of Biology and Anatomy, National Defense Medical Center, 100, Taipei, Taiwan, Republic of China aut Sun Guang-Huan Division of Urology, Department of Surgery, Tri-Service General Hospital, 100, Taipei, Taiwan, Republic of China aut Shy Shyi-Ren Department of Biology and Anatomy, National Defense Medical Center, 100, Taipei, Taiwan, Republic of China aut Shyu Hann-Yeh Department of Biology and Anatomy, National Defense Medical Center, 100, Taipei, Taiwan, Republic of China aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 189-194 0302-766X 269:2<189 1992 269 441 https://doi.org/10.1007/BF00319608 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319608 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Hwan-Wun Department of Biology and Anatomy, National Defense Medical Center, 100, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Sun Guang-Huan Division of Urology, Department of Surgery, Tri-Service General Hospital, 100, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Shy Shyi-Ren Department of Biology and Anatomy, National Defense Medical Center, 100, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Shyu Hann-Yeh Department of Biology and Anatomy, National Defense Medical Center, 100, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 189-194 0302-766X 269:2<189 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer