caa a22 4500 469084146 CHVBK 20180323133000.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319611 doi (NATIONALLICENCE)springer-10.1007/BF00319611 Bethanechol and a G-protein activator, NaF/AlCl3, induce secretory response in Paneth cells of mouse intestine [Elektronische Daten] [Y. Satoh, K. Ishikawa, Y. Oomori, S. Takeda, K. Ono] Summary: Paneth cells located at the bottom of intestinal crypts may play a role in controlling the bacterial milieu of the intestine. Using morphometry to clarify the secretory mechanism of the Paneth cells, we studied the ultrastructural changes in mouse Paneth cells produced following intra-arterial perfusion with Hanks' balanced salt solution containing a cholinergic muscarinic secretagogue (bethanechol), a neuroblocking agent (tetrodotoxin), or a G-protein activator (NAF/AlCl3). Bethanechol (2×10-4 mol/l) induced Paneth-cell secretion. Many Paneth cells massively exocytosed their secretory material into the crypt lumen; the enhanced secretion caused degranulation and vacuole formation. However, tetrodotoxin (2×10-6 mol/l) did not prevent the bethanechol-enhanced secretion by the Paneth cells. NaF (1×10-2 mol/l) and AlCl3 (1×10-5 mol/l) induced massive exocytosis of the Paneth cells; the exocytotic figures were similar to those observed in mice stimulated by bethanechol. G-protein activation was followed by a sequence of intracellular events, resulting in exocytosis. Springer-Verlag, 1992 Paneth cells nationallicence Ultrastructure nationallicence Morphometry nationallicence Bethanechol nationallicence Fluoride ion nationallicence G-protein nationallicence Mouse (Balb/c) nationallicence Satoh Y. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut Ishikawa K. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut Oomori Y. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut Takeda S. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut Ono K. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 213-220 0302-766X 269:2<213 1992 269 441 https://doi.org/10.1007/BF00319611 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319611 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Satoh Y. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut NATIONALLICENCE 700 1- Ishikawa K. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut NATIONALLICENCE 700 1- Oomori Y. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut NATIONALLICENCE 700 1- Takeda S. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut NATIONALLICENCE 700 1- Ono K. Department of Anatomy, Asahikawa, Medical College, Nishikagura 4-5, 078, Asahikawa, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 213-220 0302-766X 269:2<213 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer