caa a22 4500 469084154 CHVBK 20180323133000.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319615 doi (NATIONALLICENCE)springer-10.1007/BF00319615 Neuropeptides regulate the cardiac activity of a prosobranch mollusc, Rapana thomasiana [Elektronische Daten] [M. Fujiwara-Sakata, M. Kobayashi] Summary: Involvement of neuropeptides in the regulation of cardiac activity in a prosobranch mollusc, Rapana thomasiana, was studied physiologically as well as immunohistochemically. A catch-relaxing peptide (CARP) showed strong inhibitory effects on the heart with a lower threshold than acetylcholine. The action of CARP was in contrast to that of another neuropeptide, FMRFamide, which has previously been shown to enhance the heart beat. Benzoquinonium blocked the effects of acetylcholine and stimulation of right cardiac nerves 1 and 3b, but not those of CARP, suggesting that the effects of nerve stimulation are mainly due to the release of acetylcholine. Immunohistochemical examinations demonstrated that FMRFamide-like and CARP-like immunoreactive neurons are distributed in the visceral ganglia. Although a neuron appeared to show weak immunoreactivity to both antisera, evidence for the coexistence of peptides in a single neuron was not exhibited. Positive immunoreactivity to FMRFamide and CARP antisera also appeared in right cardiac nerves 1 and 3. In the heart, FMRFamide- and CARP-like immunoreactive fibers were restricted to the atrium and the aortic end of the ventricle, consistent with the morphological observation of innervation. The present results suggest that FMRFamide- and CARP-like peptides are involved in regulating the heart beat. Springer-Verlag, 1992 FMRFamide nationallicence Catch-relaxing peptide (CARP) nationallicence Heart beat nationallicence Immunohistochemistry- Rapana thomasiana (Mollusca) nationallicence Fujiwara-Sakata M. Physiological Laboratory, Faculty of Integrated Arts and Sciences, Hiroshima University, 1-1-89, Higashisenda-machi, Naka-ku, 730, Hiroshima, Japan aut Kobayashi M. Physiological Laboratory, Faculty of Integrated Arts and Sciences, Hiroshima University, 1-1-89, Higashisenda-machi, Naka-ku, 730, Hiroshima, Japan aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 241-247 0302-766X 269:2<241 1992 269 441 https://doi.org/10.1007/BF00319615 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319615 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujiwara-Sakata M. Physiological Laboratory, Faculty of Integrated Arts and Sciences, Hiroshima University, 1-1-89, Higashisenda-machi, Naka-ku, 730, Hiroshima, Japan aut NATIONALLICENCE 700 1- Kobayashi M. Physiological Laboratory, Faculty of Integrated Arts and Sciences, Hiroshima University, 1-1-89, Higashisenda-machi, Naka-ku, 730, Hiroshima, Japan aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 241-247 0302-766X 269:2<241 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer