caa a22 4500 469084162 CHVBK 20180323133000.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319612 doi (NATIONALLICENCE)springer-10.1007/BF00319612 Immunolocalization of inhibin α-subunit in the human testis [Elektronische Daten] A light- and electron-microscopy study [Gabriella Vannelli, Tullio Barni, Gianni Forti, Andres Negro-Vilar, Wylie Vale, Mario Serio, Giuseppe Balboni] Summary: The localization of inhibin α-subunit in the human testis was studied at the light- and electron-microscope level with immunostaining techniques. Antibodies against specific fragments of porcine and human inhibin α-subunits were utilized. At light microscopy, inhibin α-subunit immunoreactivity was detected in Sertoli cells, spermatocytes and in some Leydig cells. At electron microscopy, gold labeling was found in the cisternae of the Golgi apparatus and in the endoplasmic reticulum of Sertoli and Leydig cells. Gold labeling for inhibin was also found in coated vesicles in the cytoplasm of Sertoli cells as well as in coated pits and coated vesicles in the cytoplasm of some spermatocytes. The results of the present study suggest that, in the human testis, inhibin is produced by Sertoli and Leydig cells and is taken up by spermatocytes, on which it might act in a paracrine manner. Springer-Verlag, 1992 Testis nationallicence Inhibin nationallicence Inhibin subunits nationallicence Sertoli cells nationallicence Germ cells nationallicence Leydig cells nationallicence Human nationallicence Vannelli Gabriella Department of Human Anatomy and Histology, University of Florence, Florence, Italy aut Barni Tullio National Institute for Geriatric Studies INRCA, Florence, Italy aut Forti Gianni Department of Clinical Physiopathology, Endocrinology Unit, University of Florence, Viale Morgagni 85, I-50134, Florence, Italy aut Negro-Vilar Andres Laboratory of Molecular and Integrative Neurosciences, National Institute of Environmental Health Sciences, 27709, Research Triangle Park, NC, USA aut Vale Wylie The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute for Biological Studies, 10010 North Torrey Pines Read, 92307, La Jolla, CA, USA aut Serio Mario Department of Clinical Physiopathology, Endocrinology Unit, University of Florence, Viale Morgagni 85, I-50134, Florence, Italy aut Balboni Giuseppe Department of Human Anatomy and Histology, University of Florence, Florence, Italy aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 221-227 0302-766X 269:2<221 1992 269 441 https://doi.org/10.1007/BF00319612 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319612 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vannelli Gabriella Department of Human Anatomy and Histology, University of Florence, Florence, Italy aut NATIONALLICENCE 700 1- Barni Tullio National Institute for Geriatric Studies INRCA, Florence, Italy aut NATIONALLICENCE 700 1- Forti Gianni Department of Clinical Physiopathology, Endocrinology Unit, University of Florence, Viale Morgagni 85, I-50134, Florence, Italy aut NATIONALLICENCE 700 1- Negro-Vilar Andres Laboratory of Molecular and Integrative Neurosciences, National Institute of Environmental Health Sciences, 27709, Research Triangle Park, NC, USA aut NATIONALLICENCE 700 1- Vale Wylie The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute for Biological Studies, 10010 North Torrey Pines Read, 92307, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Serio Mario Department of Clinical Physiopathology, Endocrinology Unit, University of Florence, Viale Morgagni 85, I-50134, Florence, Italy aut NATIONALLICENCE 700 1- Balboni Giuseppe Department of Human Anatomy and Histology, University of Florence, Florence, Italy aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 221-227 0302-766X 269:2<221 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer