caa a22 4500 469084189 CHVBK 20180323133000.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00319627 doi (NATIONALLICENCE)springer-10.1007/BF00319627 Species variability in the expression of met- and leu-enkephalin-like immunoreactivity in mammalian Merkel cell dense-core granules [Elektronische Daten] A light- and electron-microscopic immunohistochemical study [S. Cheng Chew, P. Leung] Summary: Immunogold staining failed to show met-enkephalin immunoreactivity in the Merkel cell dense-core granules of rats when examined by electron microscopy, but showed gold particle staining in the Merkel cell dense-core granules of mice and nude mice. Merkel cells of hamster, guinea pig, rabbit, cat and dog were also examined using a similar method, and different antisera dilutions. Immunogold particles were consistently found in the dense-core granules of mice and nude mice at all antisera dilutions, but not in the other species, except in the dog, where a very low labelling response was encountered. Merkel cells from skin touch domes or sinus hair follicles, did not exhibit any difference in peptide expression as far as met-enkephalin immunoreactivity was concerned. In addition, all species studied, including mice and nude mice, did not show leu-enkephalin immunoreactivity in their Merkel cell dense-core granules. It is concluded that species variability in peptide expression occurs in the Merkel cell dense-core granules, and may be closely related to the different methodologies used. Springer-Verlag, 1992 Immunocytochemistry nationallicence Enkephalin nationallicence Skin nationallicence Touch dome nationallicence Merkel cells nationallicence Dense-core granules nationallicence Mammalian species nationallicence Cheng Chew S. Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T., Hong Kong aut Leung P. Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T., Hong Kong aut Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 347-351 0302-766X 269:2<347 1992 269 441 https://doi.org/10.1007/BF00319627 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00319627 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng Chew S. Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T., Hong Kong aut NATIONALLICENCE 700 1- Leung P. Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T., Hong Kong aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 269/2(1992-08-01), 347-351 0302-766X 269:2<347 1992 269 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer