caa a22 4500 469090596 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00788753 doi (NATIONALLICENCE)springer-10.1007/BF00788753 Molecular cloning of a major CENP-B epitope and its use for the detection of anticentromere autoantibodies [Elektronische Daten] [R. Verheijen, B. de Jong, E. Oberyé, W. van Venrooij] An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of anticentromere autoantibodies in sera of patients with suspected or manifest rheumatic diseases. The antigen source used in this assay consists of the recombinant protein of glutathione S-transferase (GST) fused to the last 60 C-terminal amino acid residues of the major centromere protein CENP-B. Although this CENP-B segment is only a small part of the complete polypeptide, we show that it constitutes an important autoimmune antigenic domain which is recognized by all patient sera in which ACA can be detected using the immunoblotting technique with a HeLa S3 nuclear protein extract as antigen source. Kluwer Academic Publishers, 1992 Autoantibodies nationallicence Autoimmune Epitope nationallicence CENP-B nationallicence ELISA nationallicence Verheijen R. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut de Jong B. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut Oberyé E. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut van Venrooij W. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 49-59 0301-4851 16:1<49 1992 16 11033 https://doi.org/10.1007/BF00788753 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00788753 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Verheijen R. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- de Jong B. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Oberyé E. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- van Venrooij W. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 49-59 0301-4851 16:1<49 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer