caa a22 4500 46909060X CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00788751 doi (NATIONALLICENCE)springer-10.1007/BF00788751 Histone H10 mRNA and protein accumulate early during retinoic acid induced differentiation of synchronized embryonal carcinoma cells [Elektronische Daten] [F. van Hemert, L. Jonk, O. Destrée] The very lysine-rich replacement histone variant H10 is found to be present in different murine (C1003, PC13, P19) and human (Tera-2) embryonal carcinoma cell lines. The proportion of H10 increases upon induction of differentiation of the different cell lines by various treatments. In undifferentiated PC13 EC cells H10 mRNA is present at a low level. During retinoic acid induced differentiation of mitotically synchronized PC13 EC cells, accumulation of H10 mRNA starts in the first cell cycle. The H10 protein level starts to increase in the second synchronous cycle preceding changes in the cycle parameters that become apparent in the third cycle. The results provide further support for an important role of H10 in the control of cellular differentiation in early mammalian development. Kluwer Academic Publishers, 1992 differentiation nationallicence embryonal carcinoma cells nationallicence replacement histone variant nationallicence teratocarcinoma cells nationallicence EC : embryonal carcinoma nationallicence RA : retinoic acid nationallicence DAPT : 4′-6-diamino-2-phenylindole nationallicence SDS : sodium dodecylsulphate nationallicence DMSO : dimethyl sulfoxide nationallicence TCA : trichloro acetic acid nationallicence van Hemert F. Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands aut Jonk L. Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands aut Destrée O. Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands aut Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 33-38 0301-4851 16:1<33 1992 16 11033 https://doi.org/10.1007/BF00788751 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00788751 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Hemert F. Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Jonk L. Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Destrée O. Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 33-38 0301-4851 16:1<33 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer