caa a22 4500 469090618 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00788750 doi (NATIONALLICENCE)springer-10.1007/BF00788750 Effects of the components of Newcastle disease virus on the structural order of lipid assemblies [Elektronische Daten] [Vassil Neitchev, Lubka Dumanova] The membrane M-protein of Newcastle disease virus is localized directly beneath the lipid bilayer. Although this protein is the major constituent of the virus, its structural relationship to the lipid or to the other viral component hemagglutininneuraminidase, the so called HN-glycoprotein, is still unknown. The effects of either M-protein alone or both M-protein and HN-glycoprotein on the lipid assemblies in reconstituted liposomes were determined by differential polarized phase fluorometry, steady-state fluorescence anisotropy and emission lifetime measurements. It is demonstrated that the degree of rotation of fluorophores in reconstituted liposomes is restricted by the molecular packing of lipids in the bilayer and this in turn can be correlated with the structural order of the lipids in the membrane. The experimental results show that the structural order parameters calculated from the fluorescence measurements are strongly influenced by the presence of both M-protein and HN-glycoprotein in the lipid assemblies. Kluwer Academic Publishers, 1992 fluorescence study nationallicence HN-glycoprotein nationallicence lipid assemblies nationallicence M-protein nationallicence structural order nationallicence virus nationallicence Neitchev Vassil Central Laboratory of Biophysics, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria aut Dumanova Lubka Central Veterinary Research Institute, 1000, Sofia, Bulgaria aut Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 27-31 0301-4851 16:1<27 1992 16 11033 https://doi.org/10.1007/BF00788750 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00788750 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neitchev Vassil Central Laboratory of Biophysics, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Dumanova Lubka Central Veterinary Research Institute, 1000, Sofia, Bulgaria aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 27-31 0301-4851 16:1<27 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer