caa a22 4500 469090626 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00788748 doi (NATIONALLICENCE)springer-10.1007/BF00788748 Isolation of DNA polymerase alpha from germinated wheat embryos [Elektronische Daten] [Timur Balmukhanov, Abay Erekenov, Nagima Ajtkhozhina] DNA polymerase alpha from germinated wheat embryos was purified by ammonium sulphate fractionation, chromatography on DEAE-Toyopearl, followed by phosphocellulose and heparin Sepharose columns. The specific activity of the purified enzyme was more than 60 000 units/mg. It belongs to the alpha-type according to the large molecular mass, high sensitivity to NEM, aphidicoline, 200 mM KCl, low sensitivity to ethidium bromide and the absence of inhibition by ddTTP. DNA polymerase alpha consists of four subunits as shown by SDS-PAGE and seems to be homogeneous under non-denaturating conditions. Kluwer Academic Publishers, 1992 DNA polymerase alpha nationallicence wheat embryos nationallicence ddTTP : dideoxythymidine triphosphate nationallicence NEM : N-ethylmaleimide nationallicence PMSF : phenylmethylsulfonyl fluoride nationallicence Balmukhanov Timur M.A. Ajtkhozhin Institute of Molecular Biology and Biochemistry, Kazakh Academy of Sciences, Michurina str. 86, 480012, Alma-Ata, USSR aut Erekenov Abay M.A. Ajtkhozhin Institute of Molecular Biology and Biochemistry, Kazakh Academy of Sciences, Michurina str. 86, 480012, Alma-Ata, USSR aut Ajtkhozhina Nagima M.A. Ajtkhozhin Institute of Molecular Biology and Biochemistry, Kazakh Academy of Sciences, Michurina str. 86, 480012, Alma-Ata, USSR aut Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 11-16 0301-4851 16:1<11 1992 16 11033 https://doi.org/10.1007/BF00788748 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00788748 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Balmukhanov Timur M.A. Ajtkhozhin Institute of Molecular Biology and Biochemistry, Kazakh Academy of Sciences, Michurina str. 86, 480012, Alma-Ata, USSR aut NATIONALLICENCE 700 1- Erekenov Abay M.A. Ajtkhozhin Institute of Molecular Biology and Biochemistry, Kazakh Academy of Sciences, Michurina str. 86, 480012, Alma-Ata, USSR aut NATIONALLICENCE 700 1- Ajtkhozhina Nagima M.A. Ajtkhozhin Institute of Molecular Biology and Biochemistry, Kazakh Academy of Sciences, Michurina str. 86, 480012, Alma-Ata, USSR aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 11-16 0301-4851 16:1<11 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer