caa a22 4500 469090634 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00788752 doi (NATIONALLICENCE)springer-10.1007/BF00788752 Structure and site of expression of a murine type II hair keratin [Elektronische Daten] [Edda Tobiasch, Jürgen Schweizer, Hermelita Winter] We present here a 1770 bp-long cDNA which encodes a murine type II keratin. Sequence comparisons of the keratin with those of various type II keratins expressed in mouse epidermis and internal stratified epithelia reveal that the new keratin is unrelated to epithelial keratins. Rather the structural organization of its amino- and carboxyterminal domains and the high content of cysteine and proline residues in these regions suggest that the keratin represents a murine type II hair keratin. This assumption was confirmed by in situ hybridization which localized the mRNA of the keratin in upper cells of the hair cortex and in suprabasal cells of the central core unit of filiform papillae of the tongue. Hybrid selection analyses revealed that the keratin has a molecular weight of 58 kD. It remains to be seen whether the keratin corresponds to MHb 3 or MHb 4. Kluwer Academic Publishers, 1992 mouse epithelia nationallicence keratin expression nationallicence in situ hybridization nationallicence hair keratin nationallicence Tobiasch Edda Institute of Biochemistry, German Cancer Research Center, Im Neuenheimer Feld 280, 6900, Heidelberg, Germany aut Schweizer Jürgen Institute of Biochemistry, German Cancer Research Center, Im Neuenheimer Feld 280, 6900, Heidelberg, Germany aut Winter Hermelita Institute of Biochemistry, German Cancer Research Center, Im Neuenheimer Feld 280, 6900, Heidelberg, Germany aut Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 39-47 0301-4851 16:1<39 1992 16 11033 https://doi.org/10.1007/BF00788752 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00788752 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tobiasch Edda Institute of Biochemistry, German Cancer Research Center, Im Neuenheimer Feld 280, 6900, Heidelberg, Germany aut NATIONALLICENCE 700 1- Schweizer Jürgen Institute of Biochemistry, German Cancer Research Center, Im Neuenheimer Feld 280, 6900, Heidelberg, Germany aut NATIONALLICENCE 700 1- Winter Hermelita Institute of Biochemistry, German Cancer Research Center, Im Neuenheimer Feld 280, 6900, Heidelberg, Germany aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/1(1992-02-01), 39-47 0301-4851 16:1<39 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer