caa a22 4500 469090693 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00464706 doi (NATIONALLICENCE)springer-10.1007/BF00464706 Verheijen R. Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands aut B-cell epitopes of scleroderma-specific autoantigens [Elektronische Daten] [R. Verheijen] Conclusions: It can be concluded that the precise localization of the epitopes on autoantigens associated with scleroderma has not been determined yet, and further subcloning experiments will be required to map the epitopes more precisely. However, the fact that the antigenicities of the C-terminal ends of topo I as well as of CENP-B are highly affected by the length of the fusion segments suggest that most, if not all, antigenic determinants on these parts of the autoantigens are conformational epitopes. Studies based upon molecular modelling of antibodies reacting with antigens suggest that over 90% of B-cell epitopes are conformational [50]. This implies that the most successful approach to allocate B-cell epitopes on autoantigens in the near future may be the use of techniques for mapping conformational epitopes. Such techniques are currently being developed [reviewed in 51]. Until now, the limited data available indicate that the B-cell epitopes on the scleroderma-associated autoantigens are distributed over the entire proteins. The C-terminal parts of the antigens seem to be good candidates for harboring the major autoimmune epitopes, but more experimental data will be necessary to confirm this suggestion. Kluwer Academic Publishers, 1992 autoantibody nationallicence autoantigen nationallicence epitope nationallicence scleroderma nationallicence Molecular Biology Reports Kluwer Academic Publishers 16/3(1992-06-01), 183-189 0301-4851 16:3<183 1992 16 11033 https://doi.org/10.1007/BF00464706 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00464706 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Verheijen R. Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/3(1992-06-01), 183-189 0301-4851 16:3<183 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer