caa a22 4500 469090758 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00419668 doi (NATIONALLICENCE)springer-10.1007/BF00419668 Effect of deletions 5′ to the translation initiation sequence on the expression of an mRNA in animal cells [Elektronische Daten] [M. Ganoza, N. Farrow, G. An] To learn if an mRNA·18S rRNA interaction or a special secondary structure in the mRNA start region is essential for translation in eukaryotic cells, we constructed recombinant plasmids with the SV40 early promoter 5′ to part of the Escherichia coli tuf B-lacZ gene. Deletion of bases potentially complementary to the 18S rRNA highly increased the transient β-galactosidase expressed in transfected CHO cells. Deletion of bases that fostered formation of potential hairpins with the mRNA 5′-terminus or altered the structure of the coding region reduced β-galactosidase activity suggesting that these features of the mRNA secondary structure may be essential for initiation of translation. Computer aided analysis of the potential structure of 290 mRNAs suggests these are conserved features of the initiation region. Kluwer Academic Publishers, 1992 translational initiation nationallicence 18S rRNA nationallicence mRNA secondary structure nationallicence gene expression nationallicence initiation mutants nationallicence β-galactosidase nationallicence Ganoza M. C.H. Best Institute, Banting & Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada aut Farrow N. Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada aut An G. Institute of Biological Chemistry, Washington State University, Pullman, Wash., USA aut Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 277-284 0301-4851 16:4<277 1992 16 11033 https://doi.org/10.1007/BF00419668 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00419668 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ganoza M. C.H. Best Institute, Banting & Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Farrow N. Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- An G. Institute of Biological Chemistry, Washington State University, Pullman, Wash., USA aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 277-284 0301-4851 16:4<277 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer