caa a22 4500 469090774 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00419661 doi (NATIONALLICENCE)springer-10.1007/BF00419661 Benne Rob E. C. Slater Institute for Biochemical Research, University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands aut RNA editing in trypanosomes [Elektronische Daten] The us(e) of guide RNAs [Rob Benne] Guide RNAs are encoded in maxicircle and minicircle DNA of trypanosome mitochondria. They play a pivotal role in RNA editing, a process during which the nucleotide sequence of mitochondrial RNAs is altered by U-insertion and deletion. Guide RNAs vary in length from 35 to 78 nucleotides, which correlates with the variation in length of the three functionally important regions of which they are composed: (i) a 4-14 nucleotide ‘anchor' sequence embedded in the 5′ region, which is complementary to a target sequence on the pre-edited RNA downstream of an editing domain, (ii) a middle part containing the editing information, which ranges from guiding the insertion of just one U into one site to that of the insertion of 32 Us into 10 sites, and (iii) a 5-24 nucleotide 3′ terminal oligo [U] extension. Moreover, a variable uridylation site creates gRNAs containing a varying segment of editing information for the same domain. Comparison of different guide RNAs demonstrates that, besides the U-tail, they have no obvious common primary and secondary sequence motifs, each particular sequence being unique. The occurrence in vivo and the synthesis in vitro of chimeric molecules, in which a guide RNA is covalently linked through its 3′ U-tail to an editing site of a pre-edited RNA, suggests that RNA editing occurs by consecutive transesterification reactions and is evidence that the guide RNAs not only provide the genetic information, but also the Us themselves. Kluwer Academic Publishers, 1992 guide RNA nationallicence mitochondrion nationallicence RNA editing nationallicence trypanosomes nationallicence gRNA : guide RNA nationallicence Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 217-227 0301-4851 16:4<217 1992 16 11033 https://doi.org/10.1007/BF00419661 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00419661 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Benne Rob E. C. Slater Institute for Biochemical Research, University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 217-227 0301-4851 16:4<217 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer