caa a22 4500 469090782 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00419663 doi (NATIONALLICENCE)springer-10.1007/BF00419663 Identification and typing of members of the protein-tyrosine phosphatase gene family expressed in mouse brain [Elektronische Daten] [Jan Schepens, Patrick Zeeuwen, Bé Wieringa, Wiljan Hendriks] Protein-tyrosine phosphatases (PTPases) form a novel and important class of cell regulatory proteins. We evaluated the expression of PTPases in mouse brain by polymerase chain amplification of cDNA segments that encode the catalytic domains of these enzymes. Degenerate primer pairs devised on the basis of conserved protein motifs were used to generate a series of distinct PCR-derived clones. In this way, murine homologues of the human PTPases LRP, PTPβ, PTPδ, PTPɛ and LAR were obtained. Corresponding regions in their catalytic domains were used to reveal the evolutionary relationships between all currently known mammalian PTPase protein family members. Phylogenetic reconstruction displayed considerable differences in mutation rates for closely related PTPases. Kluwer Academic Publishers, 1992 mouse brain nationallicence protein evolution nationallicence protein-tyrosine phosphatases nationallicence signal transduction nationallicence Schepens Jan Department of Cell Biology & Histology, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut Zeeuwen Patrick Department of Cell Biology & Histology, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut Wieringa Bé Department of Cell Biology & Histology, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut Hendriks Wiljan Department of Cell Biology & Histology, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 241-248 0301-4851 16:4<241 1992 16 11033 https://doi.org/10.1007/BF00419663 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00419663 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schepens Jan Department of Cell Biology & Histology, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Zeeuwen Patrick Department of Cell Biology & Histology, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Wieringa Bé Department of Cell Biology & Histology, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Hendriks Wiljan Department of Cell Biology & Histology, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 241-248 0301-4851 16:4<241 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer