caa a22 4500 469090790 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00419665 doi (NATIONALLICENCE)springer-10.1007/BF00419665 Nucleotide sequence of a pregnancy-specific β1 glycoprotein gene family member [Elektronische Daten] Identification of a functional promoter region and several putative regulatory sequences [G. Panzetta-Dutari, J. Bocco, B. Reimund, A. Flury, L. Patrito] The pregnancy-specific β1 glycoprotein (PSG) genes encode a group of heterogeneous proteins produced in large amounts by the human syncytiotrophoblast. Their expression seems to be regulated at the transcriptional level during normal pregnancy. In the present work, we isolated from a human placental library a 17 kb genomic fragment corresponding to a member of the PSG multigene family. DNA sequence analysis of 1190 nucleotides upstream of the translational start and of the first intron, revealed the presence of several putative regulatory sequences. In a transient chloramphenicol acetyltransferase expression assay, 5′ flanking sequences within 123 nucleotides upstream to the first major transcription initiation site, functioned as a strong promoter in COS-7 cells. Meanwhile, sequences 5′ further upstream had the ability to abolish this promoter activity. The sequence analyzed did not contain any obvious TATA-like boxes or G+C-rich regions, suggesting the existence of unique promoter elements implicated in transcription initiation and regulation of this PSG gene family member. Kluwer Academic Publishers, 1992 pregnancy-specific glycoproteins nationallicence placental protein nationallicence genomic clone nationallicence regulatory sequences nationallicence Panzetta-Dutari G. Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16-C.C. 61, 5016, Córdoba, Argentina aut Bocco J. Laboratoire de Génétique Moléculaire des Eucaryotes, CNRS, U 184 de Biologie Moléculaire et de Génie Génétique de l'Institut National de la Santé et la Recherche Médicale, Faculté de Médecine, 11 rue Humann, 67085, Strasbourg Cedex, France aut Reimund B. Laboratoire de Génétique Moléculaire des Eucaryotes, CNRS, U 184-INSERM, 11 rue Humann, 67085, Strasbourg Cedex, France aut Flury A. Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16-C.C. 61, 5016, Córdoba, Argentina aut Patrito L. Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16-C.C. 61, 5016, Córdoba, Argentina aut Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 255-262 0301-4851 16:4<255 1992 16 11033 https://doi.org/10.1007/BF00419665 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00419665 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Panzetta-Dutari G. Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16-C.C. 61, 5016, Córdoba, Argentina aut NATIONALLICENCE 700 1- Bocco J. Laboratoire de Génétique Moléculaire des Eucaryotes, CNRS, U 184 de Biologie Moléculaire et de Génie Génétique de l'Institut National de la Santé et la Recherche Médicale, Faculté de Médecine, 11 rue Humann, 67085, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Reimund B. Laboratoire de Génétique Moléculaire des Eucaryotes, CNRS, U 184-INSERM, 11 rue Humann, 67085, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Flury A. Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16-C.C. 61, 5016, Córdoba, Argentina aut NATIONALLICENCE 700 1- Patrito L. Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16-C.C. 61, 5016, Córdoba, Argentina aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 255-262 0301-4851 16:4<255 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer